EP 0787184 A1 19970806 - RECOMBINANT ALTERNARIA ALTERNATA ALLERGENS
Title (en)
RECOMBINANT ALTERNARIA ALTERNATA ALLERGENS
Title (de)
REKOMBINANTE ALTERNARIA ALTERNATA ALLERGENE
Title (fr)
ALLERGENES D'ALTERNARIA ALTERNATA RECOMBINES
Publication
Application
Priority
- AT 9500212 W 19951031
- AT 203894 A 19941102
Abstract (en)
[origin: WO9614407A1] The invention relates to the complete cDNA sequences of the Alternaria alternata allergenes Alt a 45 and Alt a 12. It has been possible in the course of molecular biological analysis of the allergens to identify Alt a 45 as a protein-disulphide isomerase. This protein is a 45 kD large protein to which 47 % of patients react and is thus an important primary allergen. Alt a 12 (to which 8 % of patients react) was identified as a ribosomal protein which is of interest because auto-antibodies for ribosomal proteins are found in patients suffering from lupus. It remains to be seen whether there is any connection between allergy to mould fungus and autoimmune diseases. Using this recombinant sequence and computer analysis, it has been possible to identify highly potent B- and T-cell epitopes and to use the peptides derived from the recombinant proteins for diagnosis of an allergy to Alternaria alternata mould fungus. The peptides has also proved suitable for both in vitro and in vivo allergen-specific stimulation of T-cells (proliferation, interleukin production), and for blocking the T-cells resulting in a tolerance of the allergen-specific T-lymphocytes.
[origin: WO9614407A1] The invention relates to the complete cDNA sequences of the Alternaria alternata allergenes Alt a 45 and Alt a 12. It has been possible in the course of molecular biological analysis of the allergens to identify Alt a 45 as a protein-disulphide isomerase. This protein is a 45 kD large protein to which 47 % of patients react and is thus an important primary allergen. Alt a 12 (to which 8 % of patients react) was identified as a ribosomal protein which is of interest because auto-antibodies for ribosomal proteins are found in patients suffering from lupus. It remains to be seen whether there is any connection between allergy to mould fungus and autoimmune diseases. Using this recombinant sequence and computer analysis, it has been possible to identify highly potent B- and T-cell epitopes and to use the peptides derived from the recombinant proteins for diagnosis of an allergy to Alternaria alternata mould fungus. The peptides has also proved suitable for both in vitro and in vivo allergen-specific stimulation of T-cells (proliferation, interleukin production), and for blocking the T-cells resulting in a tolerance of the allergen-specific T-lymphocytes.
IPC 1-7
C12N 15/11; C12N 15/62; C12N 15/56; C12N 15/70; C07K 14/37; G01N 33/53; A61K 39/35
IPC 8 full level
G01N 33/53 (2006.01); A61K 39/35 (2006.01); A61P 37/04 (2006.01); C07K 14/37 (2006.01); C12N 1/21 (2006.01); C12N 9/24 (2006.01); C12N 15/09 (2006.01); C12N 15/12 (2006.01); G01N 33/569 (2006.01); A61K 38/00 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP)
A61P 37/04 (2017.12); C07K 14/37 (2013.01); A61K 38/00 (2013.01); C07K 2319/00 (2013.01)
Citation (search report)
See references of WO 9614407A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9614407 A1 19960517; AT 401181 B 19960725; AT A203894 A 19951115; AU 3736795 A 19960531; CA 2204362 A1 19960517; EP 0787184 A1 19970806; FI 971894 A0 19970502; FI 971894 A 19970502; JP H10508474 A 19980825; NO 971836 D0 19970421; NO 971836 L 19970625
DOCDB simple family (application)
AT 9500212 W 19951031; AT 203894 A 19941102; AU 3736795 A 19951031; CA 2204362 A 19951031; EP 95935287 A 19951031; FI 971894 A 19970502; JP 51489096 A 19951031; NO 971836 A 19970421