EP 0791075 A2 19970827 - USE OF SPERMIDINE TO RELIEVE INHIBITION OF LIGASE CHAIN REACTION IN A CLINICAL TEST SAMPLE
Title (en)
USE OF SPERMIDINE TO RELIEVE INHIBITION OF LIGASE CHAIN REACTION IN A CLINICAL TEST SAMPLE
Title (de)
VERWENDUNG VON SPERMIDIN ZUR ENTLASTUNG VON HEMMUNG IN EINER KLINISCHEN PROBE IN EINER LIGASEKETTENREAKTION
Title (fr)
EMPLOI DE SPERMIDINE POUR ATTENUER LES EFFETS INHIBITEURS DE REACTIONS EN CHAINE DE LIGASE DANS UN ECHANTILLON D'ESSAI CLINIQUE
Publication
Application
Priority
- US 9512874 W 19951018
- US 33139194 A 19941021
- US 53221295 A 19951012
Abstract (en)
[origin: WO9612824A2] An improved method of amplifying target nucleic acids and particularly, to a method of alleviating the inhibitory effect of certain samples on ligase chain reaction amplifications. The relief of inhibition is accomplished by adding to the amplification mixture a polyamine reagent such as spermidine, in effective amounts, typically 1 to 4 nM. In other embodiments, the presence of spermidine allows for amplification to proceed in the presence of reduced concentrations of MgCl2 and ligase.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6848 (2013.01); C12Q 1/6862 (2013.01)
Citation (search report)
See references of WO 9612824A2
Designated contracting state (EPC)
AT BE CH DE ES FR GB IT LI NL
DOCDB simple family (publication)
WO 9612824 A2 19960502; WO 9612824 A3 19960627; CA 2202990 A1 19960502; EP 0791075 A2 19970827
DOCDB simple family (application)
US 9512874 W 19951018; CA 2202990 A 19951018; EP 95938175 A 19951018