EP 0799316 A1 19971008 - RECOMBINANT PROTEINASE FROM CLOSTRIDIUM HYSTOLYTICUM AND THE USE THEREOF FOR ISOLATING CELLS AND CELL GROUPS
Title (en)
RECOMBINANT PROTEINASE FROM CLOSTRIDIUM HYSTOLYTICUM AND THE USE THEREOF FOR ISOLATING CELLS AND CELL GROUPS
Title (de)
REKOMBINANTE PROTEINASE AUS CLOSTRIDIUM HISTOLYTICUM UND IHRE VERWENDUNG ZUR ISOLIERUNG VON ZELLEN UND ZELLVERBÄNDEN
Title (fr)
PROTEINASE RECOMBINEE DE CLOSTRIDIUM HISTOLYTICUM ET SON UTILISATION POUR ISOLER DES CELLULES ET DES GROUPES DE CELLULES
Publication
Application
Priority
- DE 4445891 A 19941222
- EP 9505054 W 19951220
Abstract (en)
[origin: DE4445891A1] A process is proposed for breaking down cellular tissue and releasing the constituent cells or cell groups by incubating the cellular tissue with a recombinant neutral protease from Clostridium hystolyticum. The protease in question is coded by (a) a DNA of the nucleotide 1027-1965 from SEQ ID NO:3 or by a DNA complementary to it; (b) nucleic acids which hybridise with a DNA of the nucleotide 1027-1965 from SEQ ID NO:3; (c) nucleic acids which would hybridise with one of the nucleic acids mentioned in (a) or (b) without degeneration of the genetic code. The protease is a product of a prokaryotic or eukaryotic expression of an exogenic nucleic acid. Incubation continues until the release of cells or cell groups in the desired quantity and separation of the cells or cell groups from the cellular tissue fractions.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); A61K 31/00 (2006.01); A61K 48/00 (2006.01); A61P 35/00 (2006.01); A61P 37/00 (2006.01); A61P 37/02 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/52 (2006.01); C12N 15/00 (2006.01); C12N 15/57 (2006.01); C12R 1/01 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP US)
A61P 35/00 (2017.12 - EP); A61P 37/00 (2017.12 - EP); A61P 37/02 (2017.12 - EP); C12N 9/52 (2013.01 - EP US)
Citation (search report)
See references of WO 9619583A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU NL PT SE
DOCDB simple family (publication)
DE 4445891 A1 19960627; AU 4346696 A 19960710; CA 2208324 A1 19960627; EP 0799316 A1 19971008; JP 2953477 B2 19990927; JP H10500581 A 19980120; MX 9704586 A 19971031; US 5853976 A 19981229; WO 9619583 A1 19960627
DOCDB simple family (application)
DE 4445891 A 19941222; AU 4346696 A 19951220; CA 2208324 A 19951220; EP 9505054 W 19951220; EP 95942186 A 19951220; JP 51951196 A 19951220; MX 9704586 A 19951220; US 84953697 A 19970826