EP 0804590 A1 19971105 - BIFUNCTIONAL SELECTABLE FUSION GENES BASED ON THE CYTOSINE DEAMINASE (CD) GENE
Title (en)
BIFUNCTIONAL SELECTABLE FUSION GENES BASED ON THE CYTOSINE DEAMINASE (CD) GENE
Title (de)
BIFUNKTIONELLE SELEKTIERBARE FUSIONSGENE AUF DEM CYTOSIN-DEAMINASE (CD) GEN BERUHEND
Title (fr)
GENES DE FUSION SELECTABLES ET BIFONCTIONNELS SE BASANT SUR LE GENE DE CYTOSINE-DEAMINASE (CD)
Publication
Application
Priority
- US 9405601 W 19940519
- US 6638893 A 19930521
Abstract (en)
[origin: WO9428143A1] The invention provides selectable fusion genes including a dominant positive selectable gene fused to and in reading frame with a negative selectable gene. The selectable fusion gene encodes a single bifunctional fusion protein which is capable of conferring a dominant positive selectable phenotype and a negative selectable phenotype on a cellular host. A dominant negative selectable phenotype is conferred by the cytosine deaminase (CD) gene for 5-fluorocytosine sensitivity (5-FC<s>). A dominant positive selectable phenotype is conferred, for example, by the neo gene for G-418 aminoglycoside antibiotic resistance (G-418<r>), or by the hph gene for hygromycin B resistance (Hm<r>). The present invention also provides recombinant expression vectors, such as retroviral vectors, which include selectable fusion genes, and cells transduced with the recombinant expression vectors. The bifunctional selectable fusion genes are expressed and regulated as a single genetic entity, permitting co-regulation and co-expression with a high degree of efficiency.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 5/10 (2006.01); C12N 9/12 (2006.01); C12N 9/78 (2006.01); C12N 15/55 (2006.01); C12N 15/65 (2006.01); C12N 15/867 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C12N 9/78 (2013.01); C12N 15/65 (2013.01); C12N 15/86 (2013.01); C07K 2319/00 (2013.01); C12N 2740/13043 (2013.01)
Citation (search report)
See references of WO 9428143A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9428143 A1 19941208; AU 6953394 A 19941220; CA 2163427 A1 19941208; EP 0804590 A1 19971105; JP H09500783 A 19970128
DOCDB simple family (application)
US 9405601 W 19940519; AU 6953394 A 19940519; CA 2163427 A 19940519; EP 94918041 A 19940519; JP 50078795 A 19940519