EP 0807171 A1 19971119 - SYNTHESIS OF STEREOSPECIFIC OLIGONUCLEOTIDE PHOSPHOROTHIOATES
Title (en)
SYNTHESIS OF STEREOSPECIFIC OLIGONUCLEOTIDE PHOSPHOROTHIOATES
Title (de)
HERSTELLUNG VON STEREOSPEZIFISCHEN PHOSPHOROTHIOATENOLIGONUKLEOTIDEN
Title (fr)
SYNTHESE D'OLIGONUCLEOTIDES DE PHOSPHOROTHIOATES STEREOSPECIFIQUES
Publication
Application
Priority
- US 9516086 W 19951212
- US 36263194 A 19941222
Abstract (en)
[origin: WO9619572A1] Disclosed is a method for synthesizing of stereospecific (Rp) phosphorothioate oligonucleotides. In this method, a primer comprising a plurality of deoxyribonucleotides and a ribonucleotide at the 5' terminal or 5' penultimate position, is annealed to a template. The structure is contacted with a mixture of deoxynucleoside alpha -triphosphate Sp diastereomers and a DNA polymerase to form a PS-Rp oligodeoxynucleotide extension which is liberated as a single-stranded PS-Rp oligonucleotide by cleavage after the ribonucleotide in the primer. Also disclosed are PS-Rp oligonucleotides and oligonucleotides prepared according to this method.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12N 15/113 (2013.01); C12N 15/1132 (2013.01); C12N 2310/315 (2013.01)
Citation (search report)
See references of WO 9619572A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9619572 A1 19960627; AU 4514696 A 19960710; CA 2208528 A1 19960627; CN 1175281 A 19980304; EP 0807171 A1 19971119; JP H10511267 A 19981104
DOCDB simple family (application)
US 9516086 W 19951212; AU 4514696 A 19951212; CA 2208528 A 19951212; CN 95197690 A 19951212; EP 95943748 A 19951212; JP 51985996 A 19951212