EP 0813684 B1 20020424 - DETERMINATION OF STEROIDS BY COMPETITIVE IMMUNOASSAY
Title (en)
DETERMINATION OF STEROIDS BY COMPETITIVE IMMUNOASSAY
Title (de)
BESTIMMUNG VON STEROIDEN DURCH KOMPETITIVEN IMMUNOASSAY
Title (fr)
DETERMINATION DE STEROIDES PAR DOSAGE IMMUNOLOGIQUE PAR COMPETITION
Publication
Application
Priority
- US 9602495 W 19960226
- US 39822695 A 19950303
Abstract (en)
[origin: US5932431A] A method and kit for measurement of a steroid by means of a competitive immunoassay, preferably a competitive enzyme immunoassay. The method and kit involve the use of a steroid analogue conjugated to a label. The steroids that are amenable to detection by the method and kit of the present invention include estradiol and progesterone. The method comprises the steps of: a. incubating a mixture of a test sample suspected of containing a given steroid, a solid phase coupled to an antibody specific for that steroid, and a conjugate of an analogue of that steroid to form steroid/antibody complexes and conjugate/antibody complexes on said solid phase; b. separating said solid phase from said mixture; c. measuring the amount of label present in said mixture or in said solid phase; and d. determining the amount of steroid in said sample from the amount of label. The kit comprises a solid phase coupled to an antibody specific for a steroid and a conjugate of an analogue of that steroid.
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); C07J 41/00 (2006.01); G01N 33/535 (2006.01); G01N 33/543 (2006.01); G01N 33/74 (2006.01)
CPC (source: EP US)
C07J 41/0016 (2013.01 - EP US); C07J 41/0038 (2013.01 - EP US); G01N 33/535 (2013.01 - EP US); G01N 33/743 (2013.01 - EP US); Y10S 435/806 (2013.01 - EP US); Y10S 436/906 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE ES FR GB IT LI NL
DOCDB simple family (publication)
US 5932431 A 19990803; AT E216780 T1 20020515; CA 2214085 A1 19960912; DE 69620876 D1 20020529; DE 69620876 T2 20021212; EP 0813684 A1 19971229; EP 0813684 B1 20020424; ES 2176445 T3 20021201; JP 3640970 B2 20050420; JP H11501397 A 19990202; US 5663054 A 19970902; US 6201141 B1 20010313; WO 9627800 A1 19960912
DOCDB simple family (application)
US 84078297 A 19970416; AT 96908511 T 19960226; CA 2214085 A 19960226; DE 69620876 T 19960226; EP 96908511 A 19960226; ES 96908511 T 19960226; JP 52689896 A 19960226; US 33688499 A 19990618; US 39822695 A 19950303; US 9602495 W 19960226