EP 0815260 A1 19980107 - METHOD FOR THE QUALITATIVE AND QUANTITATIVE DETECTION OF DNA DAMAGE
Title (en)
METHOD FOR THE QUALITATIVE AND QUANTITATIVE DETECTION OF DNA DAMAGE
Title (de)
VERFAHREN ZUM QUALITATIVEN UND QUANTITATIVEN NACHWEIS VON DNA-SCHÄDEN
Title (fr)
PROCEDE DE DETECTION QUALITATIVE ET QUANTITATIVE DE LESIONS DE L'ADN
Publication
Application
Priority
- FR 9600391 W 19960313
- FR 9503230 A 19950315
Abstract (en)
[origin: WO9628571A1] A method for the qualitative and quantitative detection of damages to DNA, characterised in that it includes the steps of binding the target DNA to a sensitised substrate, subjecting the DNA to the action of a test composition comprising at least one damage-generating product, subjecting the DNA to the action of a composition containing at least one cell extract active in repairing the DNA and containing markers, and detecting directly or indirectly the markers optionally incorporated into the DNA, if the repair has been effective, wherein a washing step is included between said steps and the binding and damaging steps can be reversed. The invention also provides a method for capturing the DNA directly from the cells with a lysis solution and a method for determining the repair modulation effect of some molecules. Said method is suitable for detecting DNA damage and the repair inhibition modulating activity of one or more molecules.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2018.01)
CPC (source: EP US)
C12Q 1/68 (2013.01 - EP US)
Citation (search report)
See references of WO 9628571A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9628571 A1 19960919; AU 5008996 A 19961002; CA 2215493 A1 19960919; EP 0815260 A1 19980107; FR 2731711 A1 19960920; FR 2731711 B1 19970606; JP H11510362 A 19990914; US 6261767 B1 20010717
DOCDB simple family (application)
FR 9600391 W 19960313; AU 5008996 A 19960313; CA 2215493 A 19960313; EP 96906825 A 19960313; FR 9503230 A 19950315; JP 52733996 A 19960313; US 91319397 A 19971125