EP 0817965 A1 19980114 - METHOD FOR IDENTIFYING BIOLOGICALLY ACTIVE SUBSTANCES BY THEIR EFFECT ON LIVING CELLS
Title (en)
METHOD FOR IDENTIFYING BIOLOGICALLY ACTIVE SUBSTANCES BY THEIR EFFECT ON LIVING CELLS
Title (de)
VERFAHREN ZUR IDENTIFIZIERUNG BIOLOGISCH AKTIVER SUBSTANZEN DURCH IHRE WIRKUNG AUF LEBENDEN ZELLEN
Title (fr)
PROCEDE D'IDENTIFICATION DE SUBSTANCES BIOLOGIQUEMENT ACTIVES DE PAR LEUR EFFET SUR DES CELLULES VIVANTES
Publication
Application
Priority
- DK 9600133 W 19960329
- DK 35595 A 19950331
Abstract (en)
[origin: WO9630760A1] A rapid automated method for identifying biologically active substances by their effect on living cells comprises the steps: a) culturing cells on/within a matrix that fluidically can be controlled; b) loading the cells with luminescent chemical molecules or having them previously transfected with recombinant molecules having luminescent properties; c) injecting a well defined volume of the cell carrying matrix suspension into a flow fluid which transports the suspension into a flow chamber having a wall through which the content of said flow chamber may be monitored; d) perifusing the cells with a sample containing a potentially biologically active substance; e) measuring the luminescence response of the content in the flow chamber; f) flushing the flow chamber to make it ready for a new testing cycle.
IPC 1-7
IPC 8 full level
G01N 33/15 (2006.01); C12M 1/34 (2006.01); C12Q 1/02 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP)
C12M 25/16 (2013.01); C12M 41/46 (2013.01); G01N 33/5008 (2013.01); G01N 33/502 (2013.01)
Citation (search report)
See references of WO 9630760A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE
DOCDB simple family (publication)
WO 9630760 A1 19961003; AU 5143096 A 19961016; EP 0817965 A1 19980114; JP H11504507 A 19990427
DOCDB simple family (application)
DK 9600133 W 19960329; AU 5143096 A 19960329; EP 96908028 A 19960329; JP 52881696 A 19960329