Global Patent Index - EP 0822983 A4

EP 0822983 A4 20041215 - APOPAIN

Title (en)

APOPAIN

Title (de)

APOPAIN

Title (fr)

APOPAINE

Publication

EP 0822983 A4 20041215 (EN)

Application

EP 96913801 A 19960417

Priority

  • US 9605282 W 19960417
  • US 42655795 A 19950421

Abstract (en)

[origin: WO9633268A1] The present invention is directed to an isolated and purified enzyme designated apopain, methods of using apopain to screen for compounds which modulate the activity of apopain, and compounds identified by the screens. A synthetic DNA molecule encoding full length apopain is prepared from the purified enzyme. The synthetic apopain-encoding DNA is formulated so as to optimize expression in a variety of recombinant hosts. The DNA clones produce recombinant full-length apopain and derivatives thereof. Purified native apopain and recombinant apopain are useful for identifying modulators of apopain activity and hence modifiers of pathological conditions related to the pro-inflammatory or pro-apoptotic effects of apopain. Apopain antisense molecules are useful for therapeutically reducing or eliminating the pro-inflammatory or pro-apoptotic effects of apopain, whereas gene transplantation or gene therapy with apopain is useful for enhancing the pro-inflammatory or pro-apoptotic effects of apopain. These therapies are beneficial in the treatment of immune, proliferative and degenerative diseases including, but not limited to, immune deficiency syndromes (such as AIDS), autoimmune diseases, pathogenic infections, cardiovascular and neurological injury, alopecia, aging, cancer, Parkinson's disease and Alzheimer's disease.

IPC 1-7

C12N 9/50; C12N 9/64

IPC 8 full level

A61K 31/70 (2006.01); A61K 45/00 (2006.01); A61K 48/00 (2006.01); A61P 9/00 (2006.01); A61P 25/00 (2006.01); A61P 25/28 (2006.01); A61P 31/00 (2006.01); A61P 35/00 (2006.01); A61P 37/00 (2006.01); A61P 43/00 (2006.01); C07B 61/00 (2006.01); C07K 5/113 (2006.01); C07K 16/40 (2006.01); C12N 9/64 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01)

CPC (source: EP)

A61P 9/00 (2017.12); A61P 25/00 (2017.12); A61P 25/28 (2017.12); A61P 31/00 (2017.12); A61P 35/00 (2017.12); A61P 37/00 (2017.12); A61P 43/00 (2017.12); C07K 5/1021 (2013.01); C12N 9/6475 (2013.01); A61K 48/00 (2013.01)

Citation (search report)

  • [E] WO 9625501 A2 19960822 - UNIV MICHIGAN [US], et al
  • [E] WO 9613603 A1 19960509 - HUMAN GENOME SCIENCES INC [US], et al
  • [XD] LAZEBNIK Y A ET AL: "Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE.", NATURE. 22 SEP 1994, vol. 371, no. 6495, 22 September 1994 (1994-09-22), pages 346 - 347, XP001183582, ISSN: 0028-0836
  • [XD] KAUFMANN S H ET AL: "SPECIFIC PROTEOLYTIC CLEAVAGE OF POLY(ADP-RIBOSE) POLYMERASE: AN EARLY MARKER OF CHEMOTHERAPY-INDUCED APOPTOSIS", CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, BALTIMORE, MD, US, vol. 53, no. 17, 1 September 1993 (1993-09-01), pages 3976 - 3985, XP001160867, ISSN: 0008-5472
  • [XD] THORNBERRY N A ET AL: "A NOVEL HETERODIMERIC CYSTEINE PROTEASE IS REQUIRED FOR INTERLEUKIN-1BETA PROCESSING IN MONOCYTES", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 356, 30 April 1992 (1992-04-30), pages 768 - 774, XP000986114, ISSN: 0028-0836
  • [PA] FERNANDES-ALNEMRI T ET AL: "MCH3, A NOVEL HUMAN APOPTOTIC CYSTEINE PROTEASE HIGHLY RELATED TO CPP32", CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, BALTIMORE, MD, US, vol. 55, no. 24, 15 December 1995 (1995-12-15), pages 6045 - 6052, XP000614740, ISSN: 0008-5472
  • [PX] TEWARI M ET AL: "YAMACPP32BETA, A MAMMALIAN HOMOLOG OF CED-3, IS A CRMA-INHIBITABLE PROTEASE THAT CLEAVES THE DEATH SUBSTRATE POLY(ADP-RIBOSE)POLYMERASE", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 81, 2 June 1995 (1995-06-02), pages 801 - 809, XP002013933, ISSN: 0092-8674
  • [PX] NICHOLSON D W ET AL: "IDENTIFICATION AND INHIBITION OF THE ICE/CED-3 PROTEASE NECESSARY FOR MAMMALIAN APOPTOSIS", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 376, no. 6535, 6 July 1995 (1995-07-06), pages 37 - 43, XP000574812, ISSN: 0028-0836
  • See references of WO 9633268A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

DOCDB simple family (publication)

WO 9633268 A1 19961024; CA 2218679 A1 19961024; EP 0822983 A1 19980211; EP 0822983 A4 20041215; JP H11504209 A 19990420

DOCDB simple family (application)

US 9605282 W 19960417; CA 2218679 A 19960417; EP 96913801 A 19960417; JP 53186696 A 19960417