Global Patent Index - EP 0824598 A2

EP 0824598 A2 19980225 - METHOD FOR THE SPECIFIC COUPLING OF THE CAP OF THE EXTREMITY 5' OF A FRAGMENT mRNA AND PREPARATION OF mRNA AND COMPLETE cDNA

Title (en)

METHOD FOR THE SPECIFIC COUPLING OF THE CAP OF THE EXTREMITY 5' OF A FRAGMENT mRNA AND PREPARATION OF mRNA AND COMPLETE cDNA

Title (de)

VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA

Title (fr)

METHODE DE COUPLAGE SPECIFIQUE DE LA COIFFE DE L'EXTREMITE 5' D'UN FRAGMENT D'ARNm ET PREPARATION D'ARNm ET D'ADNc COMPLET

Publication

EP 0824598 A2 19980225 (FR)

Application

EP 96914259 A 19960429

Priority

  • FR 9600651 W 19960429
  • FR 9505221 A 19950502
  • FR 9509467 A 19950803

Abstract (en)

[origin: WO9634981A2] The present invention relates to a method for the specific coupling of the cup of the extremity 5' of a eucaryotic mRNA fragment by means of a molecule functionalized by an amine function. The present invention also relates to a method for isolating the extremity 5' of mRNA and a method for the preparation of the single strand cDNA extremity complementary of the extremity 5' of mRNA and of double strand cDNA corresponding to the extremity 5' of mRNA. The present invention also discloses a method for isolating full length cDNA corresponding to the totality of mRNA.

IPC 1-7

C12Q 1/68; C12N 15/10; C12P 19/34

IPC 8 full level

C12N 15/09 (2006.01); C07H 21/02 (2006.01); C12N 15/10 (2006.01)

CPC (source: EP US)

C12N 15/1096 (2013.01 - EP US)

Citation (search report)

See references of WO 9634981A2

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9634981 A2 19961107; WO 9634981 A3 19970103; AT E227346 T1 20021115; AU 5982996 A 19961121; AU 719217 B2 20000504; CA 2220045 A1 19961107; CA 2220045 C 20011030; DE 69624695 D1 20021212; DE 69624695 T2 20030731; DK 0824598 T3 20030310; EP 0824598 A2 19980225; EP 0824598 B1 20021106; ES 2186778 T3 20030516; FR 2733762 A1 19961108; FR 2733762 B1 19970801; JP H11510364 A 19990914; PT 824598 E 20030331; US 6022715 A 20000208

DOCDB simple family (application)

FR 9600651 W 19960429; AT 96914259 T 19960429; AU 5982996 A 19960429; CA 2220045 A 19960429; DE 69624695 T 19960429; DK 96914259 T 19960429; EP 96914259 A 19960429; ES 96914259 T 19960429; FR 9509467 A 19950803; JP 53307096 A 19960429; PT 96914259 T 19960429; US 93010297 A 19971031