EP 0828841 A2 19980318 - PROCESS FOR OBTAINING ACYLOINS, PYRUVATE DECARBOXYLASES SUITABLE THEREFOR AND THEIR PRODUCTION AND DNA SEQUENCES OF THE PDC GENE CODING THEM
Title (en)
PROCESS FOR OBTAINING ACYLOINS, PYRUVATE DECARBOXYLASES SUITABLE THEREFOR AND THEIR PRODUCTION AND DNA SEQUENCES OF THE PDC GENE CODING THEM
Title (de)
VERFAHREN ZUR GEWINNUNG VON ACYLOINEN, DAFÜR GEEIGNETE PYRUVAT-DECARBOXYLASE SOWIE DEREN HERSTELLUNG UND DNA-SEQUENZ DES FÜR DIESE KODIERENDEN PDC-GENS
Title (fr)
PROCEDE DE PREPARATION D'ACYLOINES, DECARBOXYLASES DE PYRUVATE APPROPRIEES ET LEUR PREPARATION, ET SEQUENCE D'ADN DU GENE PDC LES CODANT
Publication
Application
Priority
- DE 9600928 W 19960522
- DE 19518809 A 19950526
- DE 19523269 A 19950629
Abstract (en)
[origin: US6004789A] A process for obtaining a pyruvate decarboxylase by isolation from a producer organism. The pyruvate decarboxylase is capable of forming (R)-(-)-phenylacetylcarbinole (I) in >/=95% enantiomer unit with a product ratio of (I) to 2-hydoxypropiophenone of >/=95%. In addition, the pyruvate decarboxylase has a specific activity with regard to phenylacetylcarbinole formation of >1U/mg. It is the aim of the invention to obtain a pyruvate decarboxylase with improved synthesis capacity concerning the formation of (R)-(-)-phenylacetylcarbinole. The process of the invention developed for this purpose is characterized in that use is made of a producing organism with a gene coding for pyruvate decarboxylase from Zymomonas mobilis, in the DNA sequence of which the tryptophane radical coding codon TGG is replaced at position 1174-1176 by a codon which codes for an amino acid radical with a reduced volume ratio.
IPC 1-7
IPC 8 full level
C12N 9/02 (2006.01); C12N 9/88 (2006.01); C12N 15/00 (2006.01); C12N 15/09 (2006.01); C12N 15/60 (2006.01); C12P 7/26 (2006.01); C12P 7/42 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP KR US)
C07C 49/245 (2013.01 - KR); C12N 9/88 (2013.01 - EP KR US); C12N 15/52 (2013.01 - KR); C12P 7/26 (2013.01 - EP KR US)
Citation (search report)
See references of WO 9637620A2
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
US 6004789 A 19991221; AU 5810296 A 19961211; AU 714414 B2 20000106; BR 9608798 A 19990217; CA 2222493 A1 19961128; CN 1192244 A 19980902; CZ 287619 B6 20010117; CZ 369197 A3 19980218; DE 19523269 A1 19961128; DE 19523269 C2 20000531; EP 0828841 A2 19980318; IN 186507 B 20010922; IN 189539 B 20030322; JP H11505710 A 19990525; KR 19990021965 A 19990325; WO 9637620 A2 19961128; WO 9637620 A3 19970206
DOCDB simple family (application)
US 97685297 A 19971124; AU 5810296 A 19960522; BR 9608798 A 19960522; CA 2222493 A 19960522; CN 96195936 A 19960522; CZ 369197 A 19960522; DE 19523269 A 19950629; DE 9600928 W 19960522; EP 96919594 A 19960522; IN 647KO2000 A 20001117; IN 946CA1996 A 19960524; JP 53527096 A 19960522; KR 19970708440 A 19971125