EP 0830460 A1 19980325 - SOLID PHASE SEQUENCING OF BIOPOLYMERS
Title (en)
SOLID PHASE SEQUENCING OF BIOPOLYMERS
Title (de)
FESTPHASENSEQUENZIERUNG VON BIOPOLYMEREN
Title (fr)
SEQUEN AGE DE BIOPOLYMERES EN PHASE SOLIDE
Publication
Application
Priority
- US 9605136 W 19960410
- US 41999495 A 19950411
- US 42000995 A 19950411
- US 61415196 A 19960312
Abstract (en)
[origin: WO9632504A2] This invention relates to methods for detecting and sequencing target nucleic acid sequences, and double-stranded nucleic acid sequences, to nucleic acid probes, to mass modified nucleic acid probes, to arrays of probes useful in these methods and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C07H 21/00 (2006.01); C12M 1/00 (2006.01); C12Q 1/68 (2006.01); G01N 33/566 (2006.01); H01J 49/26 (2006.01)
CPC (source: EP)
C12Q 1/6872 (2013.01); C12Q 1/6874 (2013.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9632504 A2 19961017; WO 9632504 A3 19961114; AU 5544696 A 19961030; CA 2218188 A1 19961017; EP 0830460 A1 19980325; JP H11503611 A 19990330
DOCDB simple family (application)
US 9605136 W 19960410; AU 5544696 A 19960410; CA 2218188 A 19960410; EP 96912743 A 19960410; JP 53124396 A 19960410