EP 0832209 A1 19980401 - SECRETION LEADER TRAP CLONING METHOD
Title (en)
SECRETION LEADER TRAP CLONING METHOD
Title (de)
METHODE ZUM EINFANGEN UND KLONIEREN DER ZUR FÖRDERUNG DER SEKRETION NOTWENDIGEN LEITSEQUENZEN
Title (fr)
PROCEDE DE CLONAGE PAR PIEGEAGE DE SEQUENCES LEADER DE SECRETION
Publication
Application
Priority
- US 9608936 W 19960604
- US 48349395 A 19950607
- US 47280695 A 19950607
Abstract (en)
[origin: WO9640904A1] Expression vectors and methods for cloning amino-terminal signal sequences are disclosed. These vectors and methods are useful tools for selecting, expressing and isolating previously unknown signal sequences. In turn, an orphan signal sequence can be used to clone, identify and isolate the corresponding orphan proteins that the orphan signal sequence directs to the surface of a cell. Using these expression vectors and methods, an orphan protein can be cloned in the absence of a functional assay that detects such protein.
IPC 1-7
IPC 8 full level
C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/62 (2006.01); C12P 21/02 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12N 15/1051 (2013.01); C12N 15/625 (2013.01); C12Q 1/6858 (2013.01); C07K 2319/02 (2013.01); C07K 2319/32 (2013.01); C07K 2319/90 (2013.01)
Citation (search report)
See references of WO 9640904A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9640904 A1 19961219; AU 6045196 A 19961230; CA 2223155 A1 19961219; CA 2223155 C 20030812; EP 0832209 A1 19980401; JP H11506939 A 19990622
DOCDB simple family (application)
US 9608936 W 19960604; AU 6045196 A 19960604; CA 2223155 A 19960604; EP 96918107 A 19960604; JP 50138696 A 19960604