EP 0832282 A1 19980401 - IMPROVED METHOD FOR OBTAINING FULL-LENGTH cDNA SEQUENCES
Title (en)
IMPROVED METHOD FOR OBTAINING FULL-LENGTH cDNA SEQUENCES
Title (de)
VERBESSERTER VERFAHREN ZUR HERSTELLUNG VON cDNA-SEQUENZEN MIT VOLLER LÄNGER
Title (fr)
PROCEDE AMELIORE POUR OBTENIR DES SEQUENCES D'ADN COMPLEMENTAIRE LONGUES
Publication
Application
Priority
- US 9608501 W 19960603
- US 45904695 A 19950602
- US 46235595 A 19950605
- US 48711295 A 19950607
- US 680995 P 19951115
- US 56633495 A 19951201
Abstract (en)
[origin: WO9638591A1] A method for obtaining longer cDNA sequences is provided. The method utilizes a known genomic DNA sequence or a partial cDNA sequence, such as can be obtained from GenBank partial cDNAs. Two PCR primers are designed to correspond to the ends of the known partial sequence and to anneal to DNA in a cDNA library so as to initiate extension away from the known cDNA and the other primer. The primers are added to a cDNA library with appropriate enzymes and extend through additional DNA sequence to produce PCR products, which are subsequently purified and sequenced to provide new sequences. The new sequences are then compared with the known partial cDNA sequence for areas of overlap, and the sequence is extended beyond the overlapping areas to provide longer DNA sequence.
IPC 1-7
IPC 8 full level
C07K 14/47 (2006.01); C07K 14/705 (2006.01); C12N 9/64 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01); A61K 38/00 (2006.01); A61K 48/00 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP)
C07K 14/47 (2013.01); C07K 14/705 (2013.01); C12N 9/6472 (2013.01); C12N 15/1096 (2013.01); C12Q 1/686 (2013.01); A61K 38/00 (2013.01); A61K 48/00 (2013.01); C07K 2319/00 (2013.01)
Citation (search report)
See references of WO 9638591A1
Designated contracting state (EPC)
BE DE ES FR GB IT NL
DOCDB simple family (publication)
WO 9638591 A1 19961205; AU 5972996 A 19961218; CA 2220530 A1 19961205; EP 0832282 A1 19980401; JP H11506332 A 19990608
DOCDB simple family (application)
US 9608501 W 19960603; AU 5972996 A 19960603; CA 2220530 A 19960603; EP 96917035 A 19960603; JP 53678596 A 19960603