Global Patent Index - EP 0833667 A4

EP 0833667 A4 20011121 - STABILIZATION OF POLYNUCLEOTIDE COMPLEXES

Title (en)

STABILIZATION OF POLYNUCLEOTIDE COMPLEXES

Title (de)

STABILISIERUNG VON POLYNUKLEOTIDKOMPLEXEN

Title (fr)

STABILISATION DE COMPLEXES DE POLYNUCLEOTIDES

Publication

EP 0833667 A4 20011121 (EN)

Application

EP 96916714 A 19960528

Priority

  • US 9607866 W 19960528
  • US 48543095 A 19950607

Abstract (en)

[origin: WO9640265A1] Polynucleotide complexes are stabilized by adding a cryoprotectant compound and lyophilizing the resulting formulation. Cryoprotectant compounds comprise carbohydrates, preferably lactose and sucrose, but also glucose, maltodextrins, mannitol, sorbitol, trehalose, and others. Betaines prolines, and other amino acids may also be useful. Preferably, DNA complexes are cryoprotected with lactose at concentrations of about 1.25 % to about 10 % (w/vol). Conventional buffers may also be added to the mixture. The lyophilized formulations may be stored for extended periods of time and then rehydrated prior to use.

IPC 1-7

A61K 48/00; C12Q 1/68

IPC 8 full level

C12Q 1/68 (2006.01); A61K 9/00 (2006.01); A61K 9/127 (2006.01); A61K 31/7088 (2006.01); A61K 47/26 (2006.01); A61K 47/48 (2006.01); A61K 48/00 (2006.01); C07H 21/00 (2006.01)

CPC (source: EP)

A61K 9/0075 (2013.01); A61K 9/1272 (2013.01); A61K 47/26 (2013.01); A61K 47/54 (2017.07); A61K 47/543 (2017.07); A61K 47/544 (2017.07); A61K 47/554 (2017.07); A61K 47/6911 (2017.07); A61K 47/6929 (2017.07); A61K 48/00 (2013.01)

Citation (search report)

  • [PX] WO 9627393 A1 19960912 - UNIV PITTSBURGH [US]
  • [XY] WO 9300807 A1 19930121 - CRYOLIFE INC [US]
  • [Y] WO 9512392 A1 19950511 - UNIV CALIFORNIA [US]
  • [A] WO 9219749 A1 19921112 - UNIV MICHIGAN [US], et al
  • [A] KOTANI H ET AL: "IMPROVED METHODS OF RETROVIRAL VECTOR TRANSDUCTION AND PRODUCTION FOR GENE THERAPY", HUMAN GENE THERAPY, XX, XX, vol. 5, 1994, pages 19 - 28, XP000653182, ISSN: 1043-0342
  • [A] LEGENDRE JEAN-YVES ET AL: "Cyclic amphipathic peptide-DNA complexes mediate high-efficiency transfection of adherent mammalian cells.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 90, no. 3, 1993, 1993, pages 893 - 897, XP002177989, ISSN: 0027-8424
  • [A] HAENSLER JEAN ET AL: "Polyamidoamine cascade polymers mediate efficient transfection of cells in culture.", BIOCONJUGATE CHEMISTRY, vol. 4, no. 5, 1993, pages 372 - 379, XP000395057, ISSN: 1043-1802
  • [A] KEIKO TANAKA ET AL: "CRYOPROTECTIVE MECHANISM OF SACCARIDES ON FREEZE-DRYING OF LIPOSOME", CHEMICAL AND PHARMACEUTICAL BULLETIN, PHARMACEUTICAL SOCIETY OF JAPAN. TOKYO, JP, vol. 40, no. 1, 1992, pages 1 - 5, XP000246011, ISSN: 0009-2363
  • [PX] BRUNO M S ET AL: "Cryoprotection of cationic lipid-DNA complexes for gene delivery.", PHARMACEUTICAL RESEARCH (NEW YORK), vol. 12, no. 9 SUPPL., September 1995 (1995-09-01), Annual Meeting of the American Association of Pharmaceutical Scientists;Miami Beach, Florida, USA; November 5-9, 1995, pages S79, XP001024486, ISSN: 0724-8741
  • See references of WO 9640265A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9640265 A1 19961219; AU 5938196 A 19961230; AU 707734 B2 19990715; CA 2223921 A1 19961219; EP 0833667 A1 19980408; EP 0833667 A4 20011121; EP 1491217 A1 20041229; JP 2001516329 A 20010925

DOCDB simple family (application)

US 9607866 W 19960528; AU 5938196 A 19960528; CA 2223921 A 19960528; EP 04000585 A 19960528; EP 96916714 A 19960528; JP 50079397 A 19960528