EP 0833926 A1 19980408 - CHONDROITINASE PRODUCTION IN RECOMBINANT PROTEUS VULGARIS STRAINS
Title (en)
CHONDROITINASE PRODUCTION IN RECOMBINANT PROTEUS VULGARIS STRAINS
Title (de)
HERSTELLUNG VON CHONDROITINASE IN REKOMBINANTEN PROTEUS VULGARIS STÄMMEN
Title (fr)
PRODUCTION DE CHONDROITINASE DANS LES SOUCHES DE PROTEUS VULGARIS RECOMBINEES
Publication
Application
Priority
- US 9608509 W 19960604
- US 48117995 A 19950607
Abstract (en)
[origin: WO9640938A1] Plasmids pLP<2>-1531, which encodes chondroitinases I and II, and pLP<2>-1521, which encodes chondroitinase I, are provided. These plasmids are used to transform wild-type E. coli and P. vulgaris cells. The transformed cells produce DNA encoding the production of chondroitinases I and II or chondroitinase I in the absence of natural exogenous chondroitinases I and II inducers. Additionally, the transformed P. vulgaris cells in the absence of exogenous chondroitinases I and II inducers, produce the respective enzymes in amounts in excess of those produced by wild-type P. vulgaris in the presence of exogenous chondroitinases I and II inducer(s). Starting plasmid pLP<2>-751 and intermediate plasmids pLP<2>-770, pLP<2>-1512, pLP<2>-1263, pLP<2>-1508, pLP<2>-1510, pLP<2>-1514, pLP<2>-1518, and pLP<2>-1525 are also provided. These intermediate plasmids are typically used in the preparation of pLP<2>-1531 and pLP<2>-1521. A BglII/EcoRI fragment of about 3970 bp derived from pLP<2>-1512, a EcoRI/SmaI fragment of about 2550 bp derived from pLP<2>-1514, and a BglII/SmaI(AvaI) fragment of about 6520 bp derived from pLP<2>-1518 are further disclosed. Also contemplated is the use of transformed P. vulgaris cells to produce chondroitinase I and/or II. The P. vulgaris cells above are cultured in a bacterial culture medium and in the absence of an exogenous chondroitinase I and II inducer. The cells are then harvested from the culture, and the chondroitinase I and/or II enzymes are recovered.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 1/21 (2006.01); C12N 9/16 (2006.01); C12N 15/55 (2006.01)
CPC (source: EP)
C12N 9/16 (2013.01)
Citation (search report)
See references of WO 9640938A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE
DOCDB simple family (publication)
WO 9640938 A1 19961219; AU 6033496 A 19961230; CA 2223980 A1 19961219; EP 0833926 A1 19980408; JP H11507512 A 19990706
DOCDB simple family (application)
US 9608509 W 19960604; AU 6033496 A 19960604; CA 2223980 A 19960604; EP 96917959 A 19960604; JP 50108797 A 19960604