Global patent index - EP 0838034 A4

EP 0838034 A4 2000-05-24 - IMMUNOASSAY FOR DETERMINING BLOOD CELL ACTIVATION

Title (en)

IMMUNOASSAY FOR DETERMINING BLOOD CELL ACTIVATION

Title (de)

IMMUNOASSAY ZUR BESTIMMUNG DER BLUTZELLAKTIVIERUNG

Title (fr)

DOSAGE IMMUNOLOGIQUE POUR DETERMINER L'ACTIVATION DES CELLULES DU SANG

Publication

EP 0838034 A4 (EN)

Application

EP 96918058 A

Priority

  • US 9608776 W
  • US 47352095 A

Abstract (en)

[origin: WO9641176A1] A method for determining the activation level of platelets and other types of blood cells that undergo activation by ELISA and other immunoassay techniques includes the step of reacting a sample containing the blood cells in a liquid phase with an excess quantity of an activation-specific primary antibody prior to allowing the cells in the sample to bind to a solid surface. This differs from typical ELISA procedures, wherein the cells are bound to the surface of a plastic test tube or microtiter plate prior to addition of the primary antibody. It has been discovered according to the invention that binding the cells to a surface prior to reaction with the primary antibody changes the activation level of the cells, making the assay less accurate. In addition, whole platelet ELISA, like flow cytometric analyses, can sensitively detect activated platelets.

IPC 1-7 (main, further and additional classification)

G01N 33/536; G01N 33/543

IPC 8 full level (invention and additional information)

G01N 33/569 (2006.01)

CPC (invention and additional information)

G01N 33/56966 (2013.01)

Citation (search report)

  • [X] WO 9321527 A1 19931028 - MILWAUKEE HEART RES FOUND [US]
  • [PX] US 5457028 A 19951010 - AMRANI DAVID L [US]
  • [X] SHATTIL SJ, CUNNINGHAM M, HOXIE JA: "Detection of activated platelets in blood using activation-dependent monoclonal antibodies and flow cytometry", BLOOD, vol. 70, no. 1, 1987, pages 307 - 315, XP000881391
  • [PX] AMRANI ET AL: "Development of a whole platelet ELISA to detect circulating activated platelets", J. LAB. CLIN. MED, vol. 126, no. 6, December 1995 (1995-12-01), pages 603-611, XP000881397
  • See also references of WO 9641176A1

Designated contracting state (EPC)

DE FR GB

EPO simple patent family

WO 9641176 A1 19961219; EP 0838034 A1 19980429; EP 0838034 A4 20000524

INPADOC legal status

2000-07-26 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20000106

2000-05-24 [A4] SUPPLEMENTARY SEARCH REPORT

- Ref Legal Event Code: A4

- Effective date: 20000411

2000-05-24 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): DE FR GB

1998-04-29 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 19971229

1998-04-29 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): DE FR GB