Global Patent Index - EP 0839189 A1

EP 0839189 A1 19980506 - ENZYMES AND MICROORGANISMS HAVING AMIDASE ACTIVITY FOR HYDROLYSING POLYAMIDES

Title (en)

ENZYMES AND MICROORGANISMS HAVING AMIDASE ACTIVITY FOR HYDROLYSING POLYAMIDES

Title (de)

ENZYME UND MIKROORGANISMEN MIT AMIDASEAKTIVITÄT ZUR HYDROLYSE VON POLYAMIDEN

Title (fr)

ENZYMES ET MICRO-ORGANISMES A ACTIVITE AMIDASE HYDROLYSANT LES POLYAMIDES

Publication

EP 0839189 A1 19980506 (FR)

Application

EP 96925806 A 19960717

Priority

  • FR 9601119 W 19960717
  • FR 9508916 A 19950718

Abstract (en)

[origin: FR2736927A1] An enzyme having amidase activity, in particular for substrates such as oligomers derived from PA 6.6 (Formula I) and/or PA 6 (Formula II), and characterised in that it has a ratio Ra = enzymatic activity for (poly)amide substrates of formula (I) enzymatic activity for (poly)amide substrates of formula (II) = A.E.(I) A.E.(II) that is greater than 2, preferably of at least 10 and more preferably of at least 50, is disclosed. Specifically, said enzyme consists of a peptide sequence corresponding to SEQ ID NO 2 disclosed in the description. DNA coding for the polypeptide of SEQ ID NO 2 and having a sequence corresponding to SEQ ID NO 1 disclosed in the description is also disclosed. Furthermore, microorganisms for producing said enzyme and a hydrolysis method using said enzyme and/or said microorganisms are disclosed.

IPC 1-7

C12N 9/80; C12N 15/55; C12N 1/21; C12N 1/20

IPC 8 full level

C12N 15/09 (2006.01); C12N 1/21 (2006.01); C12N 9/80 (2006.01); C12N 15/00 (2006.01); C12N 15/55 (2006.01); C12R 1/01 (2006.01); C12R 1/19 (2006.01)

CPC (source: EP US)

C12N 1/205 (2021.05 - EP US); C12N 9/80 (2013.01 - EP US); C12R 2001/15 (2021.05 - EP US)

Citation (search report)

See references of WO 9704084A1

Designated contracting state (EPC)

DE ES FR GB IT NL

DOCDB simple family (publication)

FR 2736927 A1 19970124; FR 2736927 B1 19971017; AU 6618996 A 19970218; BR 9611100 A 19991228; CN 1193347 A 19980916; EP 0839189 A1 19980506; JP H10513366 A 19981222; US 6214592 B1 20010410; WO 9704084 A1 19970206

DOCDB simple family (application)

FR 9508916 A 19950718; AU 6618996 A 19960717; BR 9611100 A 19960717; CN 96196379 A 19960717; EP 96925806 A 19960717; FR 9601119 W 19960717; JP 50636197 A 19960717; US 6498 A 19980403