EP 0842292 A1 19980520 - METHOD OF DETERMINING THE CONCENTRATION OF AN ENZYME BY PHOTOMETRIC MEASUREMENT OF THE ABSORPTION OF A DYE FORMED BY THE ENZYME IN A TEST SOLUTION
Title (en)
METHOD OF DETERMINING THE CONCENTRATION OF AN ENZYME BY PHOTOMETRIC MEASUREMENT OF THE ABSORPTION OF A DYE FORMED BY THE ENZYME IN A TEST SOLUTION
Title (de)
VERFAHREN ZUR MESSUNG DER KONZENTRATION EINES ENZYMS DURCH PHOTOMETRISCHE ABSORPTIONSMESSUNG EINES ENZYMATISCH GEBILDETEN FARBSTOFFS IN EINER MESSLÖSUNG
Title (fr)
PROCEDE PERMETTANT DE MESURER LA CONCENTRATION D'UNE ENZYME PAR MESURE DE L'ABSORPTION PHOTOMETRIQUE D'UN COLORANT FORME DE MANIERE ENZYMATIQUE DANS UNE SOLUTION DE MESURE
Publication
Application
Priority
- DE 19527160 A 19950725
- EP 9603126 W 19960716
Abstract (en)
[origin: DE19527160C1] Described is a method of determining the concentration of an enzyme by reacting the enzyme with a substrate solution and measuring the concentration of the dye thus formed by photometric measurement of the absorption of a test solution, the measurement being carried out in the presence of a second dye which is added to the test solution and which does not interfere with the measurement of the concentration of the dye formed by the enzyme. For the determination of alkaline phosphatase from a biological sample or from an enzyme immuno-assay, the preferred dye is amaranth, and it is preferably added to the test solution together with a timer solution to time the enzymatic dye-forming reaction.
IPC 1-7
IPC 8 full level
CPC (source: EP)
C12Q 1/00 (2013.01); C12Q 1/42 (2013.01); C12Q 2334/10 (2013.01)
Citation (search report)
See references of WO 9705273A1
Designated contracting state (EPC)
AT DE FR IT
DOCDB simple family (publication)
DE 19527160 C1 19970123; EP 0842292 A1 19980520; WO 9705273 A1 19970213
DOCDB simple family (application)
DE 19527160 A 19950725; EP 9603126 W 19960716; EP 96927021 A 19960716