EP 0861325 A1 19980902 - PROCESS FOR THE PREPARATION OF PEPTIDES BY WAY OF STREPTAVIDIN FUSION PROTEINS
Title (en)
PROCESS FOR THE PREPARATION OF PEPTIDES BY WAY OF STREPTAVIDIN FUSION PROTEINS
Title (de)
VERFAHREN ZUR HERSTELLUNG VON PEPTIDEN ÜBER STREPTAVIDIN-FUSIONSPROTEINE
Title (fr)
PROCEDE DE PREPARATION DE PEPTIDES PAR L'INTERMEDIAIRE DE PROTEINES DE FUSION ISSUES DE LA STREPTAVIDINE
Publication
Application
Priority
- DE 19542702 A 19951116
- EP 9604850 W 19961106
Abstract (en)
[origin: WO9718314A1] The invention relates to a process for recombinant preparation of peptides by expression of a DNA in micro-organisms, which DNA codes for a fusion protein made of streptavidin and one of the said peptides. Streptavidin and the peptide are bound by a peptide sequence which can be cleaved by an endoproteinase. The process also includes isolation of the insoluble, inactive protein, solublisation of the inactive protein using a denaturant, dilution of the denaturant at a pH value of between 8.5 and 11 until cleaving of the fusion protein by an endoproteinase can take place, cleaving of the fusion protein, lowering of the pH value until streptavin and non-cleaved fusion protein precipitate, and cleaning of the desired peptide from the supernatant. Said process is particularly suitably for producing parathromone and urodilatin and fragments thereof.
[origin: WO9718314A1] The invention relates to a process for recombinant preparation of peptides by expression of a DNA in micro-organisms, which DNA codes for a fusion protein made of streptavidin and one of the said peptides. Streptavidin and the peptide are bound by a peptide sequence which can be cleaved by an endoproteinase. The process also includes isolation of the insoluble, inactive protein, solublisation of the inactive protein using a denaturant, dilution of the denaturant at a pH value of between 8.5 and 11 until cleaving of the fusion protein by an endoproteinase can take place, cleaving of the fusion protein, lowering of the pH value until streptavin and non-cleaved fusion protein precipitate, and cleaning of the desired peptide from the supernatant. Said process is particularly suitably for producing parathromone and urodilatin and fragments thereof.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07K 14/36 (2006.01); C07K 14/58 (2006.01); C07K 14/635 (2006.01); C07K 19/00 (2006.01); C12N 15/62 (2006.01)
CPC (source: EP US)
C07K 14/36 (2013.01 - EP US); C07K 14/58 (2013.01 - EP US); C07K 14/635 (2013.01 - EP US); C12N 15/62 (2013.01 - EP US); C07K 2319/00 (2013.01 - EP US); C07K 2319/22 (2013.01 - EP US); C07K 2319/50 (2013.01 - EP US); C07K 2319/75 (2013.01 - EP US)
Citation (search report)
See references of WO 9718314A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE
DOCDB simple family (publication)
WO 9718314 A1 19970522; AU 7566396 A 19970605; CA 2237296 A1 19970522; CA 2237296 C 20081007; EP 0861325 A1 19980902; JP 2000500019 A 20000111; US 6136564 A 20001024
DOCDB simple family (application)
EP 9604850 W 19961106; AU 7566396 A 19961106; CA 2237296 A 19961106; EP 96938115 A 19961106; JP 51855197 A 19961106; US 6873898 A 19980625