EP 0862614 A1 19980909 - YEAST RECEPTOR AND G-PROTEIN FUSION PROTEIN
Title (en)
YEAST RECEPTOR AND G-PROTEIN FUSION PROTEIN
Title (de)
HEFE REZEPTOR UND G-PROTEIN FUSIONSPROTEIN
Title (fr)
RECEPTEUR DE LEVURE ET PROTEINE DE FUSION DE PROTEINE G
Publication
Application
Priority
- US 9615203 W 19960920
- US 402395 P 19950920
Abstract (en)
[origin: WO9711159A1] The invention provides protein fusions between the C-terminus of heterotrimeric G-protein-coupled receptors and the N-terminus of either wild type or mutant G-alpha proteins of the yeast Saccharomyces cerevisiae. Methods are described for creating DNA constructs that encode such fusion protein, assays for correct expression of such fusion molecules in yeast, and assays for the coupling of such fusion molecules to the pheromone-induced signal transduction pathway of yeast. Furthermore, the invention encompasses yeasts expressing the fusion proteins and methods for screening compounds for activity as agonists or antagonists of seven-transmembrane receptor function.
IPC 1-7
C12N 1/16; C12N 1/18; C12N 1/19; C12N 15/12; C12N 15/31; C12N 15/62; C12Q 1/02; G01N 33/53
IPC 8 full level
C07K 14/395 (2006.01); C07K 14/705 (2006.01); C12N 15/52 (2006.01)
CPC (source: EP)
C07K 14/395 (2013.01); C07K 14/705 (2013.01); C12N 15/52 (2013.01); C07K 2319/00 (2013.01); G01N 2333/726 (2013.01); G01N 2500/00 (2013.01)
Citation (search report)
See references of WO 9711159A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9711159 A1 19970327; AU 7115896 A 19970409; CA 2232733 A1 19970327; EP 0862614 A1 19980909
DOCDB simple family (application)
US 9615203 W 19960920; AU 7115896 A 19960920; CA 2232733 A 19960920; EP 96932305 A 19960920