EP 0865502 A4 20000816 - A FLUORESCENCE-BASED SCREENING METHOD FOR IDENTIFYING LIGANDS
Title (en)
A FLUORESCENCE-BASED SCREENING METHOD FOR IDENTIFYING LIGANDS
Title (de)
AUF FLUORESZENZ BASIERENDES ERKENNUNGSVERFAHREN FÜR LIGANDEN
Title (fr)
PROCEDE DE CRIBLAGE PAR FLUORESCENCE DESTINE A L'IDENTIFICATION DE LIGANDS
Publication
Application
Priority
- US 9619698 W 19961206
- US 834695 P 19951207
Abstract (en)
[origin: WO9720952A1] A novel method for screening chemical compounds for potential pharmaceutical effectiveness is provided. The disclosed method identifies possible therapeutic test ligands by placing them in the presence of target proteins and determining the ability of test ligands to prevent the partial unfolding of the target protein by measuring the fluorescence emission of a conformation-sensitive probe. This differs significantly from known methods of novel pharmaceutical testing in that the biochemical function of the target protein need not be known and the existence of any known ligands of the target protein is unnecessary.
IPC 1-7
IPC 8 full level
G01N 33/533 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6811 (2018.01); C12Q 1/6816 (2018.01); G01N 33/566 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
C12Q 1/6811 (2013.01); C12Q 1/6816 (2013.01); G01N 33/582 (2013.01); G01N 33/68 (2013.01)
Citation (search report)
- [Y] EP 0613007 A2 19940831 - LILLY CO ELI [US], et al
- [Y] DATABASE WPI Section Ch Week 7526, Derwent World Patents Index; Class B04, AN 75-43214w, XP002024012
- See references of WO 9720952A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9720952 A1 19970612; CA 2236339 A1 19970612; DE 865502 T1 19990819; EP 0865502 A1 19980923; EP 0865502 A4 20000816; JP 2000502440 A 20000229
DOCDB simple family (application)
US 9619698 W 19961206; CA 2236339 A 19961206; DE 96944304 T 19961206; EP 96944304 A 19961206; JP 52149297 A 19961206