EP 0873427 B8 20021106 - PROCESS FOR DETECTING AND QUANTIFYING NUCLEIC ACID MOLECULES
Title (en)
PROCESS FOR DETECTING AND QUANTIFYING NUCLEIC ACID MOLECULES
Title (de)
VERFAHREN ZUR ERFASSUNG UND QUANTIFIZIERUNG VON NUCLEINSÄURE-MOLEKÜLEN
Title (fr)
PROCEDE DE DETECTION ET DE QUANTIFICATION DE MOLECULES D'ACIDE NUCLEIQUE
Publication
Application
Priority
- DE 9700008 W 19970107
- DE 19600362 A 19960108
Abstract (en)
[origin: US6306592B1] The invention relates to a method of detecting and identifying nucleic aicd (NA) molecules within a population (I) of NA molecules of identical, similar or different sequence, in which: (a) the single-stranded or duplex ribonucleic acid (RNA) molecules or single-stranded deoxyribonucleic aicd (DNA) molecules contained in a population (I) iare convereted into a population (II) of duplex DNA molecules; b) the population (II) of duplex DNA molecules is subjected to denaturation and subsequent renaturation, so that a mixture of homo- and heteroduplex DNA molecules is present; c) heteroduplexes formed in the population (II) where unpaired of incorrectly paired nucleotides are present are reacted chemically with a carbodiimide compound; and d) the resulting carboddimide reactions products are characterized and quantified, characterized in that a chromatographic purification method is carried out between each of the steps a to d.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6827 (2018.01); G01N 33/53 (2006.01)
CPC (source: EP US)
C12Q 1/6827 (2013.01 - EP US); Y10T 436/143333 (2015.01 - EP US)
C-Set (source: EP US)
Designated contracting state (EPC)
AT BE CH DE FR GB IE LI
DOCDB simple family (publication)
US 6306592 B1 20011023; AT E222963 T1 20020915; CA 2241185 A1 19970717; DE 19600362 A1 19970710; DE 19600362 C2 19981112; DE 59708066 D1 20021002; EP 0873427 A2 19981028; EP 0873427 B1 20020828; EP 0873427 B8 20021106; JP 2000502907 A 20000314; WO 9725441 A2 19970717; WO 9725441 A3 19970925
DOCDB simple family (application)
US 10172498 A 19981202; AT 97906982 T 19970107; CA 2241185 A 19970107; DE 19600362 A 19960108; DE 59708066 T 19970107; DE 9700008 W 19970107; EP 97906982 A 19970107; JP 52474497 A 19970107