EP 0882133 A1 19981209 - REGULATION OF GENE EXPRESSION IN EUKARYOTES
Title (en)
REGULATION OF GENE EXPRESSION IN EUKARYOTES
Title (de)
REGULATION DER GENEXPRESSION IN EUKARYOTEN
Title (fr)
REGULATION DE L'EXPRESSION D'UN GENE CHEZ UN EUCARYOTE
Publication
Application
Priority
- AU 9700089 W 19970219
- AU PN816196 A 19960219
Abstract (en)
[origin: WO9730162A1] There is provided a method of regulating a eukaryotically active gene, comprising transforming a cell with a transformation cassette expressing a modulator gene product regulating the eukaryotically gene or its product and a further gene product regulating said modulator gene or its product, the promoters of two of said gene, modulator gene and further genes being selected from inducible promoters and developmental promoters for the same or complementary tissues. The lethal gene expressing barnase, a ribonuclease of B. amiloliquefaciens, is placed under the control of a tissue specific promoter, such as those derived from PrMADS1, 2 or 3 of Pinus radiata or EGM1, 2 or 3 of Eucalyptus grandis. The same tissue specific promoter is used to express Laclq gene, a repressor for barnase (barstar) being promoted by a modified 35s RNA CaMV promoter including the lac operon. The cassette is used to transform plant cells for regeneration into plants expressing the barnase in the target tissues with improved specificity and reduced promoter leakage.
IPC 1-7
IPC 8 full level
C12N 15/82 (2006.01)
CPC (source: EP)
C12N 15/8217 (2013.01); C12N 15/8231 (2013.01); C12N 15/8233 (2013.01); C12N 15/8237 (2013.01); C12N 15/8238 (2013.01); C12N 15/825 (2013.01); C12N 15/8263 (2013.01); C12N 15/8287 (2013.01)
Citation (search report)
See references of WO 9730162A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9730162 A1 19970821; AU PN816196 A0 19960314; BR 9707435 A 20000104; CA 2259456 A1 19970821; CN 1216066 A 19990505; EP 0882133 A1 19981209; JP 2000504577 A 20000418; NO 983775 D0 19980818; NO 983775 L 19981015
DOCDB simple family (application)
AU 9700089 W 19970219; AU PN816196 A 19960219; BR 9707435 A 19970219; CA 2259456 A 19970219; CN 97193833 A 19970219; EP 97904302 A 19970219; JP 52883397 A 19970219; NO 983775 A 19980818