EP 0882233 B1 20030226 - METHOD AND DEVICE FOR TREATMENT BY COMPLEXING OF A LIQUID MEDIUM
Title (en)
METHOD AND DEVICE FOR TREATMENT BY COMPLEXING OF A LIQUID MEDIUM
Title (de)
VERFAHREN UND VORRICHTUNG ZUR BEHANDLUNG DURCH KOMPLEXIERUNG EINES WÄSSRIGEN MILIEUS
Title (fr)
PROCEDE ET DISPOSITIF DE TRAITEMENT PAR COMPLEXATION D'UN MILIEU LIQUIDE
Publication
Application
Priority
- FR 9701781 W 19971006
- FR 9612331 A 19961004
Abstract (en)
[origin: US6218116B1] A method for determining the presence, in a liquid medium, of a plurality of different nucleic ligands in the free state comprises: (a) providing a support for plural complexing on which are distributed a plurality of immobilizing sites in which are immobilized a plurality of different nucleic anti-ligands; and (b) contacting the liquid medium with the support, whereby the nucleic ligands are paired with the nucleic anti-ligands. The stability of each ligand/anti-ligand complex is dependent upon at least one exogenous parameter, namely temperature, conditioning the support; the immobilizing sites on the support are differentiated, depending on the exogenous parameter, having respectively different values in the sites; and the anti-ligands are distributed among the different immobilizing sites, depending on the optimum values of the exogenous parameter, determining the stability of the different nucleic ligand/anti-ligand complexes respectively. This method may be used for sequencing nucleic acids by hybridization.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6874 (2018.01); G01N 33/543 (2006.01)
CPC (source: EP US)
C12Q 1/6874 (2013.01 - EP US); G01N 33/543 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
US 6218116 B1 20010417; AT E233404 T1 20030315; CA 2239499 A1 19980416; CA 2239499 C 20100112; DE 69719312 D1 20030403; DE 69719312 T2 20040212; EP 0882233 A1 19981209; EP 0882233 B1 20030226; FR 2754344 A1 19980410; FR 2754344 B1 19981120; JP 2000502458 A 20000229; JP 3843134 B2 20061108; WO 9815832 A1 19980416
DOCDB simple family (application)
US 7716198 A 19980626; AT 97944930 T 19971006; CA 2239499 A 19971006; DE 69719312 T 19971006; EP 97944930 A 19971006; FR 9612331 A 19961004; FR 9701781 W 19971006; JP 51725698 A 19971006