EP 0906442 A1 19990407 - GENERATION OF VIRAL TRANSFECTANTS USING RECOMBINANT DNA-DERIVED NUCLEOCAPSID PROTEINS
Title (en)
GENERATION OF VIRAL TRANSFECTANTS USING RECOMBINANT DNA-DERIVED NUCLEOCAPSID PROTEINS
Title (de)
HERSTELLUNG VON VIREN-TRANSFEKTATEN MIT VON REKOMBINANTEM DNS HERGESTELLTEN NUKLEOKAPSID-PROTEINEN
Title (fr)
PRODUCTION DE TRANSFECTANTS VIRAUX AU MOYEN DE PROTEINES NUCLEOCAPSIDIALES DERIVEES D'ADN DE RECOMBINAISON
Publication
Application
Priority
- US 9707277 W 19970430
- US 1790796 P 19960501
Abstract (en)
[origin: WO9741245A1] This invention provides a method of producing viral transfectants (viruses containing a heterologous nucleic acid) that eliminates the need for purified RNP complexes or for purified viral RNA polymerases. The method generally involves: (i) combining a ribonucleic acid (nucleic acid transcript) with an isolated nucleoprotein (NP) of the virus to form a synthetic ribonucleoprotein complex (RNP); (ii) transfecting a host cell with the synthetic ribonucleoprotein complex; and (iii) holding (culturing) the host cell under conditions permitting replication of the virus.
IPC 1-7
C12N 15/86; C12N 7/04; C12N 7/00; C07K 14/11; C12N 9/12; C12N 15/87; C12N 5/10; C12N 7/01
IPC 8 full level
C12N 15/09 (2006.01); C07K 14/11 (2006.01); C12N 5/10 (2006.01); C12N 7/00 (2006.01); C12N 7/04 (2006.01); C12N 9/12 (2006.01); C12N 15/86 (2006.01); C12N 15/87 (2006.01); A61K 39/00 (2006.01); A61K 48/00 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C12N 7/00 (2013.01); C12N 9/127 (2013.01); C12N 15/86 (2013.01); C12N 15/87 (2013.01); A61K 39/00 (2013.01); A61K 48/00 (2013.01); C12N 2710/14143 (2013.01); C12N 2760/16122 (2013.01); C12N 2760/16162 (2013.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9741245 A1 19971106; AU 2749297 A 19971119; CA 2253595 A1 19971106; EP 0906442 A1 19990407; JP 2000509280 A 20000725
DOCDB simple family (application)
US 9707277 W 19970430; AU 2749297 A 19970430; CA 2253595 A 19970430; EP 97921462 A 19970430; JP 53919897 A 19970430