Global Patent Index - EP 0912722 A4

EP 0912722 A4 20011205 - METHOD OF CONSTRUCTING VECTORS FOR HOMOLOGOUS RECOMBINATION DIRECTED MUTAGENESIS

Title (en)

METHOD OF CONSTRUCTING VECTORS FOR HOMOLOGOUS RECOMBINATION DIRECTED MUTAGENESIS

Title (de)

VERFAHREN ZUR KONSTRUKTION VON VEKTOREN ZUR HOMOLOGEN REKOMBINATION DURCH GERICHTETE MUTAGENESE

Title (fr)

PROCEDE SERVANT A CONSTRUIRE DES VECTEURS DE RECOMBINAISON HOMOLOGUE POUR UNE MUTAGENESE DIRIGEE

Publication

EP 0912722 A4 20011205 (EN)

Application

EP 98908603 A 19980220

Priority

  • DE 19707012 A 19970221
  • US 9803243 W 19980220

Abstract (en)

[origin: WO9837175A1] The present invention provides a novel vector system and thereby a novel method for the simplified construction of recombinant vectors for directed mutagenesis. Said vector system is used to modify the eukaryotic genome, particularly of embryonic stem cells, at precise and predefined loci by the means of homologous recombination. Furthermore, said system finds its usage in the generation of new strategies for gene therapy and in the generation of genetically modified higher eukaryotic organisms.

IPC 1-7

C12N 5/00; C12N 15/00; C12N 15/69

IPC 8 full level

C12N 15/09 (2006.01); C12N 15/90 (2006.01)

CPC (source: EP)

C12N 15/907 (2013.01); A01K 2217/05 (2013.01)

Citation (search report)

  • [A] WO 9514769 A1 19950601 - UNIV SOUTH FLORIDA [US]
  • [Y] NEHLS M ET AL: "Two large insert vectors, lambda PS and lambda KO, facilitate rapid mapping and targeted disruption of mammalian genes", BIOTECHNIQUES, vol. 17, no. 4, October 1994 (1994-10-01), pages 770 - 775, XP002179274
  • [DY] STORCK T ET AL: "Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse", NUCLEIC ACIDS RESEARCH., vol. 24, no. 22, 15 November 1996 (1996-11-15), pages 4594 - 4596, XP002076777
  • [PX] TSUZUKI T ET AL: "Embryonic stem cell gene targeting using bacteriophage lambda vectors generated by phage-plasmid recombination", NUCLEIC ACIDS RESEARCH., vol. 26, no. 4, 15 February 1998 (1998-02-15), pages 988 - 993, XP002179275
  • [T] WATTLER S ET AL: "Construction of gene targeting vectors from lambda KOS genomic libraries", BIOTECHNIQUES, vol. 26, no. 6, June 1999 (1999-06-01), pages 1150-1156, 1158, 1160, XP002179272
  • See references of WO 9837175A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9837175 A1 19980827; EP 0912722 A1 19990506; EP 0912722 A4 20011205; JP 2002514072 A 20020514

DOCDB simple family (application)

US 9803243 W 19980220; EP 98908603 A 19980220; JP 53687598 A 19980220