EP 0914615 A1 19990512 - PROCESS FOR ESTABLISHING DIRECT DIAGNOSTIC EVIDENCE OF PATHOGENIC, GENETICALLY-CONDITIONED POINT MUTATIONS
Title (en)
PROCESS FOR ESTABLISHING DIRECT DIAGNOSTIC EVIDENCE OF PATHOGENIC, GENETICALLY-CONDITIONED POINT MUTATIONS
Title (de)
VERFAHREN ZUM DIREKTEN DIAGNOSTISCHEN NACHWEIS VON PATHOGENEN, GENETISCH BEDINGTEN PUNKTMUTATIONEN
Title (fr)
PROCEDE POUR ETABLIR DIRECTEMENT LA PREUVE DIAGNOSTIQUE DE MUTATIONS PONCTUELLES PATHOGENES D'ORDRE GENETIQUE
Publication
Application
Priority
- DE 19624802 A 19960621
- EP 9703241 W 19970620
Abstract (en)
[origin: DE19624802A1] The invention relates to a rapid process for medical and diagnostic, qualitative and quantitative protein analysis of the exchange of individual amino acids with pathogenic or non-pathogenic effects on the organism. The medical and diagnostic analysis is achieved by combination of enzymatic or chemical separation of the isolated peptide, chromatographic separation of the fragments and analysis by mass spectrometry, and of direct liquid chromatography-mass spectrometry (LC/MS) and indirect matrix-assisted laser desorption ionisation mass spectrometry (MALDI-MS), and analysis by capillary electrophoresis. By comparing the protein samples of healthy people with the samples of sick people, said process can be used to determine novel, presently unknown mutations, and to quantify the expression and incorporation of a wild type to form a mutant.
IPC 1-7
IPC 8 full level
C12Q 1/04 (2006.01); G01N 33/68 (2006.01); G01N 30/72 (2006.01)
CPC (source: EP US)
G01N 33/6818 (2013.01 - EP US); G01N 33/6887 (2013.01 - EP US); G01N 30/7233 (2013.01 - EP US); G01N 2333/4712 (2013.01 - EP US); Y10S 435/968 (2013.01 - EP US)
Citation (search report)
See references of WO 9749993A1
Designated contracting state (EPC)
AT CH DE FR GB IT LI
DOCDB simple family (publication)
DE 19624802 A1 19980102; EP 0914615 A1 19990512; JP 2000513439 A 20001010; US 6326163 B1 20011204; WO 9749993 A1 19971231
DOCDB simple family (application)
DE 19624802 A 19960621; EP 9703241 W 19970620; EP 97929238 A 19970620; JP 50231498 A 19970620; US 14740499 A 19990406