EP 0923736 A1 19990623 - USE OF A CARRIER MATERIAL FOR SAMPLE PREPARATION AND DETECTION FOR A GENETIC-ENGINEERING ANALYSIS PROCESS
Title (en)
USE OF A CARRIER MATERIAL FOR SAMPLE PREPARATION AND DETECTION FOR A GENETIC-ENGINEERING ANALYSIS PROCESS
Title (de)
VERWENDUNG EINES TRÄGERMATERIALS ZUR PROBENVORBEREITUNG UND DETEKTION BEI GENTECHNISCHEN ANALYSENVERFAHREN
Title (fr)
UTILISATION D'UN MATERIAU SUPPORT POUR LA PREPARATION ET LA DETECTION D'ECHANTILLONS DANS UN PROCEDE D'ANALYSE EN GENIE GENETIQUE
Publication
Application
Priority
- DE 19625211 A 19960625
- EP 9703332 W 19970625
Abstract (en)
[origin: WO9749992A1] The invention relates to the use of a carrier material in the form of a moulded structure, loose feed of the sorption material in the form of particles, in the form of a gel or a dispersion for preparation and detection of samples which are examined by a genetic-engineering analysis process. The carrier material has at least one constituent for carrying out solid phase assays, is permeable to a fluid, has an average pore diameter of 0.1 to 100 mu m and no or a very low unspecified sorption capacity for affinity materials. The carrier material is standardised so that the component present in a certain unit volume thereof for carrying out solid phase assays (affinity material) binds its complementary constituent, during single homogenous passage of a certain amount of a diluted solution of the complementary constituent at a particular concentration, specifically at an amount which varies by a charging value of +/- 40 % between a plurality of statistically relevant charging operations. The bound amount remains constant in the said range for a plurality of subsequent flow operations of suitable fluids on the carrier material such as blocking of specified or unspecified adsorption points, washing of the carrier material charged with the constituent for carrying out the solid phase assay, and delivery of affinity-reactive materials.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6806 (2018.01); G01N 33/543 (2006.01); G01N 33/544 (2006.01); G01N 33/545 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP)
C12Q 1/6806 (2013.01); G01N 33/543 (2013.01); G01N 33/544 (2013.01); G01N 33/545 (2013.01)
Citation (search report)
See references of WO 9749992A1
Designated contracting state (EPC)
BE CH DE ES FI FR GB IE IT LI NL SE
DOCDB simple family (publication)
WO 9749992 A1 19971231; AU 3344597 A 19980114; AU 3436897 A 19980114; EP 0923736 A1 19990623; JP 2000514644 A 20001107; WO 9749991 A1 19971231
DOCDB simple family (application)
EP 9703332 W 19970625; AU 3344597 A 19970625; AU 3436897 A 19970625; EP 9703331 W 19970625; EP 97930402 A 19970625; JP 50235398 A 19970625