EP 0927268 A1 19990707 - APPARATUS AND METHOD FOR PERFORMING SEQUENCING OF NUCLEIC ACID POLYMERS
Title (en)
APPARATUS AND METHOD FOR PERFORMING SEQUENCING OF NUCLEIC ACID POLYMERS
Title (de)
VORRICHTUNG UND VERFAHREN ZUR SEQUENZIERUNG VON NUKLEINSÄUREN
Title (fr)
APPAREIL ET PROCEDE POUR LA MISE EN OEUVRE D'UN SEQUEN AGE DE POLYMERES NUCLEOTIDIQUES
Publication
Application
Priority
- US 9715056 W 19970827
- US 70371096 A 19960827
Abstract (en)
[origin: WO9808978A1] An apparatus for processing samples containing DNA to produce a sequencing fragment mixture comprises a sample processing element comprising: a thermocycling region having one or more chambers for receiving a DNA sequencing reaction mixture and forming sequencing fragments therefrom; a separation region comprising a separation matrix for separating the sequencing fragments formed in the thermocycling regions; a detection region for detection of the separated sequencing fragments; and means for regulating the temperature in the thermocycling region of the sample processing element to provide a plurality of thermal cycles, each cycle including at least a denaturation phase and an extension phase. The apparatus for processing sample can be placed in a holder which is associated with means for applying an electric field to the separation region of a sample processing apparatus placed within the holder to cause polynucleotide sequencing fragments to migrate through the separation region from the thermocycling region to the detection region; and means for detecting polynucleotide fragments within the detection region of the sample processing apparatus placed within the holder.
IPC 1-7
IPC 8 full level
B01L 7/00 (2006.01); G01N 27/447 (2006.01)
CPC (source: EP)
B01L 7/52 (2013.01); G01N 27/44743 (2013.01)
Citation (search report)
See references of WO 9808978A1
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
WO 9808978 A1 19980305; AU 4236597 A 19980319; CA 2264961 A1 19980305; EP 0927268 A1 19990707
DOCDB simple family (application)
US 9715056 W 19970827; AU 4236597 A 19970827; CA 2264961 A 19970827; EP 97940630 A 19970827