EP 0927762 B1 20040107 - Method for removing fumarase activity, Microorganisms obtainable by the method and production of optically active aminopolycarboxylic acids using the microorganisms
Title (en)
Method for removing fumarase activity, Microorganisms obtainable by the method and production of optically active aminopolycarboxylic acids using the microorganisms
Title (de)
Verfahren zum Entfernen von Fumarase Aktivität, durch diese Verfahren erhältliche Mikroorganismen und Herstellung von optisch aktiven Aminopolycarbonsäuren mit diesen Mikroorganismen
Title (fr)
Méthode d'enlever de l'activité de fumarase,microorganismes obtenus par cette méthode et production d'acides aminopolycarboxyliques optiquement actives par ces microorganismes
Publication
Application
Priority
JP 31104697 A 19971028
Abstract (en)
[origin: EP0927762A2] This invention relates to a method for removing fumarase activity from a microorganism or processed product thereof having ethylenediamine-N,N'-disuccinic acid ethylenediamine lyase activity, which includes treating the microorganism or processed product thereof with an aqueous alkaline solution at a pH of 8.0 to 10.5 in the presence of at least one salt with a concentration of 5 mM to 1000 mM. The salt is preferably selected from the group consisting of sodium, potassium, ammonium and C2-6 alkanediamine salts of boric acid, phosphoric acid, hydrochloric acid, sulfuric acid, acetic acid, oxalic acid, fumaric acid, maleic acid and ethylenediamine-N,N'-disuccinic acid, and mixtures thereof. This invention also relates to a microorganism or processed product thereof having reduced fumarase activity obtainable by the above described method, and to a method for producing an optically active aminopolycarboxylic acid from fumaric acid and a compound having amino group in the presence of the microorganim or processed product thereof having reduced fumarase activity as a catalyst.
IPC 1-7
IPC 8 full level
C12N 1/38 (2006.01); C12N 9/00 (2006.01); C12N 9/99 (2006.01); C12P 13/04 (2006.01)
CPC (source: EP US)
C12N 1/38 (2013.01 - EP US); C12N 9/93 (2013.01 - EP US); C12N 9/99 (2013.01 - EP US); C12P 13/04 (2013.01 - EP US); Y10S 435/80 (2013.01 - EP US); Y10S 435/82 (2013.01 - EP US); Y10S 435/822 (2013.01 - EP US); Y10S 435/83 (2013.01 - EP US); Y10S 435/848 (2013.01 - EP US); Y10S 435/874 (2013.01 - EP US)
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
EP 0927762 A2 19990707; EP 0927762 A3 20000119; EP 0927762 B1 20040107; DE 69820975 D1 20040212; DE 69820975 T2 20050127; US 6103508 A 20000815
DOCDB simple family (application)
EP 98308810 A 19981028; DE 69820975 T 19981028; US 17996698 A 19981028