EP 0929573 A1 19990721 - METHODS FOR THE ISOLATION AND PROLIFERATION OF INSULIN-SECRETING CELLS
Title (en)
METHODS FOR THE ISOLATION AND PROLIFERATION OF INSULIN-SECRETING CELLS
Title (de)
METHODEN FÜR DIE ISOLIERUNG UND VERMEHRUNG VON INSULIN-SEKRETIERENDEN ZELLEN.
Title (fr)
PROCEDES DESTINES A L'ISOLATION ET A LA PROLIFERATION DE CELLULES SECRETANT DE L'INSULINE
Publication
Application
Priority
- US 9711018 W 19970624
- US 2080196 P 19960624
Abstract (en)
[origin: WO9749728A1] Methods have been developed for isolating and proliferating insulin-secreting mammalian cells. Invention methods involve isolating insulin-secreting cells and preparing a primary culture in which insulin is secreted under the control of one or more insulin promoter transcription factors, e.g., NKX 6.1, HNF3-beta, STF-1, Beta 2, and the like. Such proliferated insulin-secreting cells are distinguished from all other cells in that they are proliferated and in that they have measurable intracellular levels of one or more transcription factors associated with insulin production, as can readily be determined by suitable assays.
IPC 1-7
C07K 14/47; C07K 14/475; C12N 5/00; C12N 5/02; C12N 5/08; C12P 21/02; C12Q 1/02; C12Q 1/04
IPC 8 full level
C12N 5/071 (2010.01); C12Q 1/24 (2006.01)
CPC (source: EP)
C12N 5/0676 (2013.01); C12Q 1/24 (2013.01)
Citation (search report)
See references of WO 9749728A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9749728 A1 19971231; AU 3410497 A 19980114; AU 724745 B2 20000928; CA 2258499 A1 19971231; EP 0929573 A1 19990721; JP 2000513227 A 20001010
DOCDB simple family (application)
US 9711018 W 19970624; AU 3410497 A 19970624; CA 2258499 A 19970624; EP 97930221 A 19970624; JP 50349698 A 19970624