EP 0929661 A4 20000112 - NOVEL METHOD OF CULTURING HUMAN EPITHELIAL CELLS FOR THE IDENTIFICATION OF CANCER THERAPEUTICS AND DIAGNOSTICS

Title (en)

NOVEL METHOD OF CULTURING HUMAN EPITHELIAL CELLS FOR THE IDENTIFICATION OF CANCER THERAPEUTICS AND DIAGNOSTICS

Title (de)

NEUARTIGES VERFAHREN DER KULTIVIERUNG MENSCHLICHER EPITHELZELLENZUR IDENTIFIZIERUNG VON THERAPEUTIKA UND DIAGNOSTIKA GEGEN KREBS

Title (fr)

NOUVEAUX PROCEDES DE MISE EN CULTURE DE CELLULES EPITHELIALES HUMAINES POUR L'IDENTIFICATION D'UNE THERAPEUTIQUE ET D'UN DIAGNOSTIC DU CANCER

Publication

EP 0929661 A4 20000112 (EN)

Application

EP 96940533 A 19961114

Priority

US 9618538 W 19961114
US 55833195 A 19951115

Abstract (en)

[origin: WO9718296A1] Methods are provided for obtaining primary cultures of human breast, colonic and prostate epithelial cells using a fibroblast co-culture tissue culture system. The methods can be used to obtain normal, precancerous and malignant epithelial cell cultures. Methods are also provided for deriving substantially pure epithelial cell strains from the primary cultures, and for immortalizing the cell strains to provide cell lines. The primary cultures, cell strains and cell lines are used in biological and biochemical in vitro screening and/or diagnostic assays.

IPC 1-7

C12N 5/00

IPC 8 full level

C12N 5/06 (2006.01); C12N 5/071 (2010.01); C12N 5/09 (2010.01)

CPC (source: EP)

C12N 5/0631 (2013.01); C12N 5/0679 (2013.01); C12N 5/0693 (2013.01); C12N 2500/25 (2013.01); C12N 2501/11 (2013.01); C12N 2501/39 (2013.01); C12N 2502/13 (2013.01); C12N 2502/30 (2013.01); C12N 2503/00 (2013.01); C12N 2510/04 (2013.01)

Citation (search report)

[DXY] WO 9015862 A1 19901227 - CORNELL RES FOUNDATION INC [US]
[DY] US 4423145 A 19831227 - STAMPFER MARTHA R [US], et al
[X] WO 8808448 A2 19881103 - BAY MICHAEL [CH]
[A] WO 9321529 A1 19931028 - UNIV DUKE [US], et al
[A] WO 9213103 A1 19920806 - UNIV JOHNS HOPKINS [US], et al
[A] EP 0117486 A2 19840905 - SLOAN KETTERING INST CANCER [US]
[A] WO 9321958 A1 19931111 - UNIV GEORGETOWN [US]
See references of WO 9718296A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI NL PT SE

DOCDB simple family (publication)

WO 9718296 A1 19970522; AU 7739296 A 19970605; CA 2237391 A1 19970522; EP 0929661 A1 19990721; EP 0929661 A4 20000112

DOCDB simple family (application)

US 9618538 W 19961114; AU 7739296 A 19961114; CA 2237391 A 19961114; EP 96940533 A 19961114