EP 0929679 A2 19990721 - HISTIDINE-TAGGED SHIGA TOXINS, TOXOIDS, AND PROTEIN FUSIONS WITH SUCH TOXINS AND TOXOIDS, METHODS FOR THE PURIFICATION AND PREPARATION THEREOF
Title (en)
HISTIDINE-TAGGED SHIGA TOXINS, TOXOIDS, AND PROTEIN FUSIONS WITH SUCH TOXINS AND TOXOIDS, METHODS FOR THE PURIFICATION AND PREPARATION THEREOF
Title (de)
HISTIDIN-MARKIERUNG VON SHIGA-TOXINEN, TOXOIDEN UND VON FUSIONSPROTEINEN VON DIESEN TOXINEN UND TOXOIDEN UND METHODEN FÜR IHRE AUFREINIGUNG UND PRÄPARATION
Title (fr)
TOXINES ET TOXOIDES SHIGA A MARQUES PAR L'HISTIDINE, FUSIONS DE PROTEINES AVEC CES TOXINES ET TOXOIDES, ET LEURS PROCEDES DE PURIFICATION ET DE PREPARATION
Publication
Application
Priority
- US 9715836 W 19970909
- US 2563796 P 19960910
Abstract (en)
[origin: WO9811229A2] The present invention describes the isolation and purification of biologically and immunologically active histidine-tagged Shiga toxins (His-tagged), a toxin associated with HC and the potentially life-threatening sequela HUS transmitted by strains of pathogenic bacteria. The present invention describes how his-tagging greatly simplifies and expedites purifying Shiga toxins, and describes an improved method for such purification. One aspect of the invention is obtaining and using Shiga toxoids that are immunoreactive but not toxic. Another aspect of the invention is obtaining and using fusion proteins of His-tagged Shiga toxins or toxoids. Yet another aspect of the invention is obtaining and using antibodies to His-gagged Shiga toxins, toxoids, or Shiga toxin/toxoid fusion proteins.
IPC 1-7
C12N 15/31; C12N 15/62; C07K 14/245; C07K 14/25; C07K 16/12; A61K 39/40; A61K 48/00
IPC 8 full level
C12N 15/09 (2006.01); A61K 31/711 (2006.01); A61K 39/395 (2006.01); A61K 48/00 (2006.01); A61P 31/04 (2006.01); C07K 1/22 (2006.01); C07K 14/245 (2006.01); C07K 14/25 (2006.01); C07K 16/12 (2006.01); C12N 1/21 (2006.01); C12N 15/31 (2006.01); A61K 39/00 (2006.01)
CPC (source: EP US)
A61P 31/04 (2018.01 - EP); C07K 14/245 (2013.01 - EP); C07K 14/25 (2013.01 - EP); A61K 39/00 (2013.01 - EP US); A61K 2039/51 (2013.01 - EP); C07K 2319/00 (2013.01 - EP)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9811229 A2 19980319; WO 9811229 A3 19980416; AR 010218 A1 20000607; AU 4184597 A 19980402; CA 2265887 A1 19980319; EP 0929679 A2 19990721; JP 2001500730 A 20010123
DOCDB simple family (application)
US 9715836 W 19970909; AR P970104115 A 19970909; AU 4184597 A 19970909; CA 2265887 A 19970909; EP 97939845 A 19970909; JP 51374898 A 19970909