EP 0932337 A4 19991229 - VITRIFICATION SOLUTIONS FOR SHELF PRESERVATION OF CELLS, TISSUES, ORGANS AND ORGANISMS
Title (en)
VITRIFICATION SOLUTIONS FOR SHELF PRESERVATION OF CELLS, TISSUES, ORGANS AND ORGANISMS
Title (de)
VITRIFIZIERUNGSLÖSUNGEN ZUR LANGFRISTIGEN AUFBEWAHRUNG VON ZELLEN, GEWEBEN, ORGANEN UND ORGANISMEN
Title (fr)
SOLUTIONS DE VITRIFICATION SERVANT A CONSERVER A LONG TERME DES CELLULES, DES TISSUS, DES ORGANES ET DES ORGANISMES
Publication
Application
Priority
- US 9715611 W 19970905
- US 2557096 P 19960906
Abstract (en)
[origin: WO9809514A1] The method of preservation by vitrification, described in the present application, provides for storage of samples at higher temperatures than in conventional methods and can be applied to cells, multicellular tissues, organs and organismes. The method of the present invention includes preparing a solution of vitrification non-permeating co-solutes (amino acids, betaines, carbohydrates, or other non-permeating co-solutes that effectively decrease the chemical potential of permeating cryoprotectants in aqueous solutions), a permeating cryoprotectant and a non-permeating cryoprotectant (polyvinylpyrrolidone, polyethylene glycol, dextran, hydroxy ethyl starch, Ficol, etc.), contacting a sample with the vitrification solution and storing the sample at a storage temperature. The method also includes the step of rehydrating the preserved sample in a rehydration solution prepared in the manner of the vitrification storage solution. The present invention is also directed to a vitrification solution and a rehydration solution as described in connection with the method.
IPC 1-7
IPC 8 full level
CPC (source: EP)
A01N 1/02 (2013.01); A01N 1/0221 (2013.01); C12N 1/04 (2013.01)
Citation (search report)
- [XY] US 4559298 A 19851217 - FAHY GREGORY M [US]
- [X] EP 0475409 A2 19920318 - LIFECELL CORP [US]
- [XY] US 5145770 A 19920908 - TUBO ROSS A [US], et al
- [X] WO 9413135 A1 19940623 - DEV BIOTECHNOLOGICAL PROCESSES [IT], et al
- [Y] BEARLEY C A ET AL: "Optimal concentrations for non-penetrating cryopreservatives of human blood", J. PHARM. PHARMACOLOGY, & 124TH BRITISH PHARMACEUTICAL CONFERENCE, MANCHESTER, 14-17.09.1987, vol. 39, 1987, XP002120780
- [A] DATABASE MEDLINE [online] BOURNE W M: "Antigenicity of cryopreserved corneas", XP002120781, Database accession no. 1574 & ARCHIVES OF OPHTHALMOLOGY, vol. 93, no. 3, pages 215 - 218, ISSN: 0003-9950
- See references of WO 9809514A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9809514 A1 19980312; AU 4252097 A 19980326; EP 0932337 A1 19990804; EP 0932337 A4 19991229; IL 128855 A0 20000131; JP 2001502664 A 20010227
DOCDB simple family (application)
US 9715611 W 19970905; AU 4252097 A 19970905; EP 97940831 A 19970905; IL 12885597 A 19970905; JP 51291698 A 19970905