EP 0938586 A1 19990901 - KINASE ACTIVITY MEASUREMENT USING FLUORESCENCE POLARIZATION
Title (en)
KINASE ACTIVITY MEASUREMENT USING FLUORESCENCE POLARIZATION
Title (de)
MESSUNG DER KINASEAKTIVITÄT MITTELS FLUORESZENTPOLARISATION
Title (fr)
MESURE DE L'ACTIVITE DE LA KINASE PAR POLARISATION DE FLUORESCENCE
Publication
Application
Priority
- US 9719570 W 19971028
- US 2983196 P 19961028
Abstract (en)
[origin: WO9818956A1] A method for quantitating enzyme activity of phosphorylation and dephosphorylation of a peptide or protein substrate, comprising measuring the fluorescence polarization of a fluorescence-emitting reporter molecule in solution with the phosphate. Then, adding an enzyme, either a kinase or a phosphatase, and incubating the solution. Finally, measuring the fluorescence polarization of the solution after the enzyme has had an opportunity to react with the peptide or protein substrate.
IPC 1-7
IPC 8 full level
G01N 33/542 (2006.01); G01N 33/573 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
G01N 33/542 (2013.01); G01N 33/573 (2013.01); G01N 33/68 (2013.01); G01N 33/6842 (2013.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB IE IT LI NL SE
DOCDB simple family (publication)
WO 9818956 A1 19980507; EP 0938586 A1 19990901; EP 0938586 A4 20020130
DOCDB simple family (application)
US 9719570 W 19971028; EP 97952171 A 19971028