EP 0941314 A1 19990915 - TARGET NUCLEIC ACID SEQUENCE AMPLIFICATION METHOD
Title (en)
TARGET NUCLEIC ACID SEQUENCE AMPLIFICATION METHOD
Title (de)
VERFAHREN ZUR AMPLIFIZIERUNG VON ZIELNUKLEINSÄURESEQUENZEN
Title (fr)
PROCEDE D'AMPLIFICATION D'UNE SEQUENCE D'UN ACIDE NUCLEIQUE CIBLE
Publication
Application
Priority
- FR 9701445 W 19970801
- FR 9609977 A 19960802
Abstract (en)
[origin: WO9805766A1] A target nucleic acid sequence amplification method wherein at least the target nucleic acid sequence, at least one oligonucleotide primer specific for the target sequence, one or more enzymatic activities and nucleotides are provided, and the target sequence is amplified under conditions suitable in particular for said enzymatic or activity or activities, is disclosed. According to the method, at least one of the nucleotides is a prefunctionalised nucleotide that differs from the other nucleotides at least in that it includes at least one unprotected reactive covalence function in at least one predetermined site of the base of said nucleotide, in order to give a prefunctionalised amplification product including at least one such prefunctionalised nucleotide. Nucleotides for carrying out the method are also disclosed.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07H 19/06 (2006.01); C07H 21/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12N 15/10 (2006.01); C12P 19/30 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01); C12Q 1/686 (2018.01)
CPC (source: EP US)
C07H 19/06 (2013.01 - EP US); C07H 21/00 (2013.01 - EP US); C12N 15/10 (2013.01 - EP US); C12P 19/30 (2013.01 - EP US); C12P 19/34 (2013.01 - EP US); C12Q 1/686 (2013.01 - EP US)
Citation (search report)
See references of WO 9805766A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9805766 A1 19980212; CA 2262019 A1 19980212; CA 2262019 C 20111115; EP 0941314 A1 19990915; JP 2000515382 A 20001121; JP 4211948 B2 20090121; US 6537783 B1 20030325
DOCDB simple family (application)
FR 9701445 W 19970801; CA 2262019 A 19970801; EP 97936732 A 19970801; JP 50768198 A 19970801; US 21465899 A 19990111