Global Patent Index - EP 0953040 A4

EP 0953040 A4 2001-12-12 - SKIN REGENERATION USING MESENCHYMAL STEM CELLS

Title (en)

SKIN REGENERATION USING MESENCHYMAL STEM CELLS

Title (de)

HAUTREGENRATION UNTER VERWENDUNG VON MESENCHYMALER STAMMZELLEN

Title (fr)

REGENERATION DE LA PEAU AU MOYEN DES CELLULES SOUCHES MESENCHYMATEUSES

Publication

EP 0953040 A4 (EN)

Application

EP 97922401 A

Priority

  • US 9706760 W
  • US 1631796 P

Abstract (en)

[origin: WO9741208A1] Disclosed is a multilayer skin equivalent having (i) a scaffold layer incorporated with dermis-forming cells, and (ii) a keratinocyte layer. Also disclosed is a multilayer dermal equivalent having at least one of (i) a first layer of at least one skin-associated extracellular matrix component containing papillary dermal fibroblasts; and, in laminar relationship therewith, (ii) a second layer of at least one skin-associated extracellular matrix component containing reticular dermal fibroblasts. Preferred embodiments include those where the scaffold or dermis-forming layer is a multilayer selected from the group consisting of (a) a layer containing isolated papillary dermis-forming cells and a layer containing isolated reticular dermis-forming cells; (b) a layer containing isolated papillary dermis-forming cells and a layer containing isolated, culture expanded mesenchymal stem cells; and (c) a layer containing isolated reticular dermis-forming cells and a layer containing isolated, culture expanded mesenchymal stem cells. Also enclosed is a multilayer skin equivalent having (i) a scaffold layer comprising a first layer of at least one skin-associated extracellular matrix component containing papillary dermal fibroblasts and, in laminar relationship therewith, a second layer of at least one skin-associated extracellular matrix component containing reticular dermal fibroblasts; and (ii) a keratinocyte layer. Also disclosed is an injectable composition of dermis-forming cells and at least one skin-associated extracellular matrix component in a pharmaceutically acceptable injectable carrier. The injectable composition can further include keratinocytes.

IPC 1-7 (main, further and additional classification)

C12N 5/00; C12N 11/00; C12N 11/02; A61F 2/10; C12N 5/06

IPC 8 full level (invention and additional information)

A61F 2/10 (2006.01); A61L 27/00 (2006.01); A61L 27/38 (2006.01); A61L 27/60 (2006.01); C12N 5/071 (2010.01); C12N 5/077 (2010.01); A61K 35/12 (2015.01)

CPC (invention and additional information)

A61L 27/3891 (2013.01); A61L 27/3804 (2013.01); A61L 27/3813 (2013.01); A61L 27/3834 (2013.01); A61L 27/3886 (2013.01); A61L 27/3895 (2013.01); A61L 27/60 (2013.01); C12N 5/0656 (2013.01); C12N 5/0698 (2013.01); A61K 35/12 (2013.01); C12N 2502/094 (2013.01); C12N 2502/1323 (2013.01); C12N 2502/1358 (2013.01); C12N 2533/54 (2013.01)

Citation (search report)

  • [X] EP 0358506 A2 19900314 - MARROW TECH INC [US]
  • [X] AUGER F A ET AL: "SKIN EQUIVALENT PRUDUCED WITH HUMAN COLAGEN", IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL, TISSUE CULTURE ASSOCIATION, COLUMBIA, MD, US, vol. 31, no. 6, June 1995 (1995-06-01), pages 432 - 439, XP001021117, ISSN: 1071-2690
  • [X] GRACTER L J ET AL: "CHARACTERIZATION OF LABEL-RETAINING CELLS IN THE EPIDERMIS OF A HUMAN SKIN EQUIVALENT", CELL PROLIFERATION, OXFORD, GB, vol. 29, no. 12, 1996, pages 679 - 688, XP001021103, ISSN: 0960-7722
  • See also references of WO 9741208A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9741208 A1 19971106; AU 2808397 A 19971119; CA 2253724 A1 19971106; EP 0953040 A1 19991103; EP 0953040 A4 20011212; JP 2000508922 A 20000718; US 6497875 B1 20021224

INPADOC legal status


2002-09-04 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20020516

2001-12-12 [A4] DESPATCH OF SUPPLEMENTARY SEARCH REPORT

- Effective date: 20011031

2001-12-12 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A4

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-12-12 [RIC1] CLASSIFICATION (CORRECTION)

- Free text: 7C 12N 5/00 A, 7C 12N 11/00 B, 7C 12N 11/02 B, 7A 61F 2/10 B, 7C 12N 5/06 B

1999-11-03 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19981020

1999-11-03 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE