EP 0953040 A4 20011212 - SKIN REGENERATION USING MESENCHYMAL STEM CELLS
Title (en)
SKIN REGENERATION USING MESENCHYMAL STEM CELLS
Title (de)
HAUTREGENRATION UNTER VERWENDUNG VON MESENCHYMALER STAMMZELLEN
Title (fr)
REGENERATION DE LA PEAU AU MOYEN DES CELLULES SOUCHES MESENCHYMATEUSES
Publication
Application
Priority
- US 9706760 W 19970424
- US 1631796 P 19960426
Abstract (en)
[origin: WO9741208A1] Disclosed is a multilayer skin equivalent having (i) a scaffold layer incorporated with dermis-forming cells, and (ii) a keratinocyte layer. Also disclosed is a multilayer dermal equivalent having at least one of (i) a first layer of at least one skin-associated extracellular matrix component containing papillary dermal fibroblasts; and, in laminar relationship therewith, (ii) a second layer of at least one skin-associated extracellular matrix component containing reticular dermal fibroblasts. Preferred embodiments include those where the scaffold or dermis-forming layer is a multilayer selected from the group consisting of (a) a layer containing isolated papillary dermis-forming cells and a layer containing isolated reticular dermis-forming cells; (b) a layer containing isolated papillary dermis-forming cells and a layer containing isolated, culture expanded mesenchymal stem cells; and (c) a layer containing isolated reticular dermis-forming cells and a layer containing isolated, culture expanded mesenchymal stem cells. Also enclosed is a multilayer skin equivalent having (i) a scaffold layer comprising a first layer of at least one skin-associated extracellular matrix component containing papillary dermal fibroblasts and, in laminar relationship therewith, a second layer of at least one skin-associated extracellular matrix component containing reticular dermal fibroblasts; and (ii) a keratinocyte layer. Also disclosed is an injectable composition of dermis-forming cells and at least one skin-associated extracellular matrix component in a pharmaceutically acceptable injectable carrier. The injectable composition can further include keratinocytes.
IPC 1-7
IPC 8 full level
A61F 2/10 (2006.01); A61L 27/00 (2006.01); A61L 27/38 (2006.01); A61L 27/60 (2006.01); C12N 5/06 (2006.01); C12N 5/071 (2010.01); C12N 5/077 (2010.01); C12N 5/08 (2006.01); A61K 35/12 (2006.01)
CPC (source: EP US)
A61L 27/3804 (2013.01 - EP US); A61L 27/3813 (2013.01 - EP US); A61L 27/3834 (2013.01 - EP US); A61L 27/3886 (2013.01 - EP US); A61L 27/3891 (2013.01 - EP US); A61L 27/3895 (2013.01 - EP US); A61L 27/60 (2013.01 - EP US); C12N 5/0656 (2013.01 - EP US); C12N 5/0698 (2013.01 - EP US); A61K 35/12 (2013.01 - EP US); C12N 2502/094 (2013.01 - EP US); C12N 2502/1323 (2013.01 - EP US); C12N 2502/1358 (2013.01 - EP); C12N 2533/54 (2013.01 - EP)
Citation (search report)
- [X] EP 0358506 A2 19900314 - MARROW TECH INC [US]
- [X] AUGER F A ET AL: "SKIN EQUIVALENT PRUDUCED WITH HUMAN COLAGEN", IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL, TISSUE CULTURE ASSOCIATION, COLUMBIA, MD, US, vol. 31, no. 6, June 1995 (1995-06-01), pages 432 - 439, XP001021117, ISSN: 1071-2690
- [X] GRACTER L J ET AL: "CHARACTERIZATION OF LABEL-RETAINING CELLS IN THE EPIDERMIS OF A HUMAN SKIN EQUIVALENT", CELL PROLIFERATION, OXFORD, GB, vol. 29, no. 12, 1996, pages 679 - 688, XP001021103, ISSN: 0960-7722
- See references of WO 9741208A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9741208 A1 19971106; AU 2808397 A 19971119; CA 2253724 A1 19971106; EP 0953040 A1 19991103; EP 0953040 A4 20011212; JP 2000508922 A 20000718; US 6497875 B1 20021224
DOCDB simple family (application)
US 9706760 W 19970424; AU 2808397 A 19970424; CA 2253724 A 19970424; EP 97922401 A 19970424; JP 53900197 A 19970424; US 17144598 A 19981026