EP 0954570 A1 19991110 - A RECOMBINANT ENZYME WITH MUTANASE ACTIVITY
Title (en)
A RECOMBINANT ENZYME WITH MUTANASE ACTIVITY
Title (de)
Rekombinantes Enzym mit Mutanaseaktivität
Title (fr)
ENZYME RECOMBINANTE A ACTIVITE MUTANASE
Publication
Application
Priority
- DK 9700283 W 19970630
- DK 71596 A 19960628
- DK 100496 A 19960917
Abstract (en)
[origin: WO9800528A1] The present invention relates to method for constructing an expression vector comprising a mutanase gene obtained from a filamentous fungus suitable for heterologous production comprising the steps of a) isolating a DNA sequence encoding a mutanase from a filamentous fungus, b) introducing a kex2-site or kex2-like site between the DNA sequences encoding the pro-peptide and the mature region of the mutanase, c) cloning the DNA sequence obtained in step b) into a suitable expression vector. The invention also relates to a recombinant expression vector comprising said mutanase gene sequence and a kex2 cleavage site between the DNA sequence encoding the pro-peptide and the region encoding the mature mutanase, a filamentous fungus host cell, a process for producing recombinant mutanase and a recombinant mutanase. It is also the object of the invention to provide compositions useful in oral care products for humans and animals.
IPC 1-7
IPC 8 full level
A23K 1/165 (2006.01); A23L 29/00 (2016.01); A61K 8/00 (2006.01); A61K 8/66 (2006.01); A61K 8/99 (2006.01); A61Q 11/00 (2006.01); C12N 1/15 (2006.01); C12N 9/24 (2006.01); C12N 15/09 (2006.01); C12N 15/56 (2006.01); A61K 38/00 (2006.01); C12R 1/69 (2006.01); C12R 1/885 (2006.01)
CPC (source: EP)
A23K 20/189 (2016.05); A23L 29/06 (2016.07); A61K 8/66 (2013.01); A61Q 11/00 (2013.01); C12N 9/2402 (2013.01); C12Y 302/01084 (2013.01); A61K 38/00 (2013.01); A61K 2800/86 (2013.01); C07K 2319/02 (2013.01)
Citation (search report)
See references of WO 9800528A1
Designated contracting state (EPC)
BE CH DE ES FR GB IT LI NL
DOCDB simple family (publication)
WO 9800528 A1 19980108; AU 3254497 A 19980121; AU 721693 B2 20000713; CA 2258291 A1 19980108; CN 1226282 A 19990818; EP 0954570 A1 19991110; JP 2000514288 A 20001031
DOCDB simple family (application)
DK 9700283 W 19970630; AU 3254497 A 19970630; CA 2258291 A 19970630; CN 97196831 A 19970630; EP 97928131 A 19970630; JP 50376398 A 19970630