EP 0954591 A2 19991110 - MINI-ADENOVIRAL VECTOR
Title (en)
MINI-ADENOVIRAL VECTOR
Title (de)
MINI-ADENOVIRALER VEKTOR
Title (fr)
VECTEUR MINI-ADENOVIRAL
Publication
Application
Priority
- US 9710218 W 19970530
- US 65896196 A 19960531
- US 79121897 A 19970131
Abstract (en)
[origin: WO9745550A2] This invention is related to adenoviral (Ad) vectors and their applications in the field of genetic medicine, including gene transfer, gene therapy, and gene vaccination. More specifically, this invention is related to the Ad vectors that carry the minimal cis-element of the Ad genome (mini-Ad vector) and are capable of delivering transgenes and/or heterologous DNA up to 36 kb. The generation and propagation of the mini-Ad vectors require trans-complementation of a packaging-attenuated and replication-defective helper Ad (helper) in an Ad helper cell line. This invention further comprises a methodology for generating a mini-adenoviral (mini-Ad) vector for use in gene therapy of hemophilia and animal test systems for in vivo evaluation of the Ad vectors. More specifically, this invention describes factor VIII (FVIII) Ad vectors that only contain minimal cis-element of the Ad genome (so-called mini-Ad) and comprise a human FVIII cDNA with other supporting DNA elements up to 36 kb. The FVIII mini-Ad can be generated and preferentially amplified through the assistance of a packaging-attenuated helper Ad and a helper cell line. This invention also reports designs and methods for producing transgenic mouse models that can be used for in vivo testing the mini-Ad.
IPC 1-7
C12N 15/86; C12N 15/34; C12N 5/10; C12N 7/01; A61K 35/76; A01K 67/027
IPC 8 full level
A01K 67/027 (2006.01); A61K 31/711 (2006.01); A61K 35/76 (2006.01); A61K 38/43 (2006.01); A61K 48/00 (2006.01); A61P 7/04 (2006.01); C07K 14/755 (2006.01); C12N 5/10 (2006.01); C12N 7/00 (2006.01); C12N 15/09 (2006.01); C12N 15/34 (2006.01); C12N 15/85 (2006.01); C12N 15/861 (2006.01); C12Q 1/02 (2006.01); C12Q 1/68 (2006.01); G01N 21/78 (2006.01); G01N 33/53 (2006.01); G01N 33/68 (2006.01); A61K 38/00 (2006.01)
CPC (source: EP)
A01K 67/0278 (2013.01); A61P 7/04 (2017.12); C07K 14/755 (2013.01); C12N 15/8509 (2013.01); C12N 15/86 (2013.01); A01K 2207/15 (2013.01); A01K 2217/00 (2013.01); A01K 2217/05 (2013.01); A01K 2217/075 (2013.01); A01K 2217/20 (2013.01); A01K 2267/0306 (2013.01); A61K 38/00 (2013.01); A61K 48/00 (2013.01); C12N 2710/10343 (2013.01); C12N 2800/108 (2013.01); C12N 2800/30 (2013.01); C12N 2810/859 (2013.01); C12N 2830/003 (2013.01); C12N 2830/008 (2013.01); C12N 2830/38 (2013.01); C12N 2830/85 (2013.01); C12N 2840/20 (2013.01); C12N 2840/203 (2013.01); C12N 2840/206 (2013.01)
Citation (search report)
See references of WO 9745550A2
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9745550 A2 19971204; WO 9745550 A3 19980409; CA 2257148 A1 19971204; EP 0954591 A2 19991110; JP 2001502883 A 20010306; JP 2005000172 A 20050106
DOCDB simple family (application)
US 9710218 W 19970530; CA 2257148 A 19970530; EP 97928961 A 19970530; JP 2004199828 A 20040706; JP 54309597 A 19970530