EP 0956356 A1 19991117 - -i(CANDIDA UTILIS) TRANSFORMATION SYSTEM
Title (en)
-i(CANDIDA UTILIS) TRANSFORMATION SYSTEM
Title (de)
CANDIDA UTILIS TRANSFORMATIONSSYSTEM
Title (fr)
SYSTEME DE TRANSFORMATION DANS -i(CANDIDA UTILIS)
Publication
Application
Priority
- CU 9700005 W 19971003
- CU 1996082 A 19961003
Abstract (en)
[origin: WO9814600A1] The present invention discloses a transformation system useful to express heterologous proteins in the Candida utilis yeast, based on obtaining auxotrophic mutants of said species as well as the isolation of different genes, from a genomic library, which complement said auxotrophies. The transformation system uses as hosts new auxotrophic mutants obtained from the yeast NRRL Y-1084 of Candida utilis which are defective mainly in the biosynthetic ways of uracyl and histidine, which are transformed with plasmids containing as selection markers the genes URA3 and HIS3 of Candida utilis. Another aspect of the invention is the isolation of the gene coding for the enzyme sucrose invertase or beta -fructofuranosidase of Candida utilis, as well as the identification of sequences for promoting, secretion signalling and termination of said gene INV1. These sequences are useful to obtain the expression of heterologous proteins in said yeast.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 1/16 (2006.01); C12N 1/19 (2006.01); C12N 9/24 (2006.01); C12N 15/00 (2006.01); C12N 15/81 (2006.01); C12R 1/72 (2006.01)
CPC (source: EP)
C12N 9/2431 (2013.01); C12N 15/81 (2013.01); C12Y 302/01026 (2013.01)
Citation (search report)
See references of WO 9814600A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9814600 A1 19980409; AU 4548597 A 19980424; AU 744698 B2 20020228; BR 9713313 A 20001024; CA 2268004 A1 19980409; CN 1237208 A 19991201; CU 22722 A1 20020228; EP 0956356 A1 19991117; JP 2001501475 A 20010206; RU 2235127 C2 20040827
DOCDB simple family (application)
CU 9700005 W 19971003; AU 4548597 A 19971003; BR 9713313 A 19971003; CA 2268004 A 19971003; CN 97199610 A 19971003; CU 1996082 A 19961003; EP 97943729 A 19971003; JP 51608598 A 19971003; RU 99109095 A 19971003