Global Patent Index - EP 0968301 A2

EP 0968301 A2 20000105 - TRANSGLYCOLYTIC SYNTHESIS OF BRANCHED OLIGOSACCHARIDES

Title (en)

TRANSGLYCOLYTIC SYNTHESIS OF BRANCHED OLIGOSACCHARIDES

Title (de)

TRANSGLYKOLYTISCHE SYNTHESE VERZWEIGTER OLIGOSACCHARIDE

Title (fr)

SYNTHESE TRANSGLYCOLYTIQUE D'OLIGOSACCHARIDES RAMIFIES

Publication

EP 0968301 A2 20000105 (EN)

Application

EP 98913699 A 19980311

Priority

  • EP 9801393 W 19980311
  • IT MI970566 A 19970313

Abstract (en)

[origin: WO9840512A2] The invention relates to a process exclusively relying on transglycolytic enzymes, for the synthesis of branched oligosaccharides. According to the described process, a donor saccharide is first incubated with an acceptor saccharide and with a suitable glycosidase. The resulting di- or oligosaccharide is then incubated with a second, different glycosidase and with a donor saccharide, to form a second glucosidic bond differing from the one formed in the first stage, thereby obtaining a branched oligosaccharide. The process is entirely and exclusively performed in water phase and allows to obtain, with high yield and specificity, branched oligosaccharides such as the trisaccharide Lewis-X.

IPC 1-7

C12P 19/14; C07H 3/06; C12N 9/24; C12N 9/38

IPC 8 full level

C07H 3/06 (2006.01); C12P 19/14 (2006.01)

CPC (source: EP)

C07H 3/06 (2013.01); C12P 19/14 (2013.01)

Citation (search report)

See references of WO 9840512A3

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB IE IT LI NL PT SE

DOCDB simple family (publication)

WO 9840512 A2 19980917; WO 9840512 A3 19981210; AU 6830198 A 19980929; EP 0968301 A2 20000105; IT 1290071 B1 19981019; IT MI970566 A1 19980914

DOCDB simple family (application)

EP 9801393 W 19980311; AU 6830198 A 19980311; EP 98913699 A 19980311; IT MI970566 A 19970313