EP 0968424 A1 20000105 - DIAGNOSTIC NEODYMIUM(III), YTTERBIUM(III), OR ERBIUM(III) ION-LIGAND COMPLEXES
Title (en)
DIAGNOSTIC NEODYMIUM(III), YTTERBIUM(III), OR ERBIUM(III) ION-LIGAND COMPLEXES
Title (de)
ION-LIGAND KOMPLEXE AUF NEODYM (III), YTTERBIUM (III) ODER ERBIUM (III) IN DER DIAGNOSTIK
Title (fr)
COMPLEXES DE LIGANDS-IONS DE NEODYMIUM (III), YTTERBIUM (III) OU ERBIUM (III)
Publication
Application
Priority
- EP 98913667 A 19980228
- EP 9801287 W 19980228
- EP 97200615 A 19970303
- US 4235497 P 19970324
Abstract (en)
[origin: WO9839654A2] The invention relates to a method for detection of an analyte in a test sample by a specific binding reaction among the analyte, a specific binding partner for the analyte, and an (immuno)reactant provided with a label, characterized in that the label is a lanthanide ion-ligand complex wherein the lanthanide ion is neodymium(III) ion (Nd<3+>), ytterbium(III) ion (Yb<3+>), or erbium(III) ion (Er<3+>) and the ligand comprises or is in contact with a sensitizing moiety which absorbs in the 400-1000 nm region, and preferably in the 400-800 nm region. Further, a diagnostic kit is disclosed as well as a method of detecting an analyte in a matrix of biomedical interest through an oligonucleotide, an antigen, or an antibody attached to a material, preferably core-shell latex or with specific binding sites wherein the antigen or antibody is labeled with the lanthanide ion-ligand complex and brought into contact with the analyte, after which the analyte with the lanthanide-ion complex is immobilized on the material, and, optionally, residual lanthanide-ion complex is removed, after which the sample obtained is irradiated with light in the 400-1000 nm region, and the emitted light from said sample is detected if said analyte is present in the matrix of biomedical interest.
IPC 1-7
IPC 8 full level
G01N 33/533 (2006.01)
CPC (source: EP)
G01N 33/533 (2013.01); G01N 2458/40 (2013.01); Y10S 435/968 (2013.01); Y10S 435/969 (2013.01); Y10S 435/971 (2013.01)
Citation (search report)
See references of WO 9839654A2
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB IE IT LI NL
DOCDB simple family (publication)
WO 9839654 A2 19980911; WO 9839654 A3 20000106; EP 0968424 A1 20000105
DOCDB simple family (application)
EP 9801287 W 19980228; EP 98913667 A 19980228