EP 0972067 A1 20000119 - METHOD FOR REVERSIBLE IMMOBILIZING OLIGO- AND/OR POLYSACCHARIDES
Title (en)
METHOD FOR REVERSIBLE IMMOBILIZING OLIGO- AND/OR POLYSACCHARIDES
Title (de)
VERFAHREN ZUM REVERSIBLEN IMMOBILISIEREN VON OLIG- ODER POLYSACCHARIDEN
Title (fr)
PROCEDE D'IMMOBILISATION REVERSIBLE D'OLIGO ET/OU DE POLYSACCHARIDES
Publication
Application
Priority
- EP 98912344 A 19980220
- EP 9800967 W 19980220
- EP 97102951 A 19970222
Abstract (en)
[origin: WO9837222A1] A method for the preparation of oligo- and/or polysaccharides, which are essentially homogenous with respect to the length of a carbohydrate chain and/or the respective molecular weight, from a sample having precursors of the oligo- and/or polysaccharide to be prepared, comprising oligo- and/or polysaccharides which are heterogenous with respect to the length of the carbohydrate chain and/or their respective molecular weight or do not have the appropriate length or weight, comprising the steps of: immobilizing precursors of the oligo- and/or polysaccharides with a carbohydrate chain present in a sample, by reversible binding the oligo- and/or polysaccharides on a surface having at least one domain for a specific interaction with said oligo- and/or polysaccharides, the at least one domain being capable of interacting at least with parts of the precursor of the oligo- and/or polysaccharide chain by contacting the sample with said surface and forming a complex between at least one domain and the precursor of the oligo- and/or polysaccharides; treating said complex with an agent for cleaving the carbohydrate chain selectively at the site of the domains; removing the cleaved product and release the oligo- and/or polysaccharides bound to the domain, said oligo- and/or polysaccharides having an essentially homogenous length of the carbohydrate chain or an essentially homogenous molecular weight.
IPC 1-7
C12P 19/04; C12P 19/26; C08B 37/08; A61K 31/715; C08L 5/08; G01N 33/68; C07K 17/00
IPC 8 full level
A61K 31/715 (2006.01); C07K 14/47 (2006.01); C07K 17/00 (2006.01); C08B 37/08 (2006.01); C08L 5/08 (2006.01); C12P 19/04 (2006.01); C12P 19/26 (2006.01); G01N 33/66 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
C07K 14/4725 (2013.01); C08B 37/0072 (2013.01); C12P 19/04 (2013.01); C12P 19/26 (2013.01); G01N 33/66 (2013.01); C07K 2319/00 (2013.01)
Citation (search report)
See references of WO 9837222A1
Designated contracting state (EPC)
AT BE CH DE ES FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 9837222 A1 19980827; EP 0861903 A1 19980902; EP 0972067 A1 20000119
DOCDB simple family (application)
EP 9800967 W 19980220; EP 97102951 A 19970222; EP 98912344 A 19980220