Global patent index - EP 0972067 A1

EP 0972067 A1 2000-01-19 - METHOD FOR REVERSIBLE IMMOBILIZING OLIGO- AND/OR POLYSACCHARIDES

Title (en)

METHOD FOR REVERSIBLE IMMOBILIZING OLIGO- AND/OR POLYSACCHARIDES

Title (de)

VERFAHREN ZUM REVERSIBLEN IMMOBILISIEREN VON OLIG- ODER POLYSACCHARIDEN

Title (fr)

PROCEDE D'IMMOBILISATION REVERSIBLE D'OLIGO ET/OU DE POLYSACCHARIDES

Publication

EP 0972067 A1 (EN)

Application

EP 98912344 A

Priority

  • EP 98912344 A
  • EP 9800967 W
  • EP 97102951 A

Abstract (en)

[origin: WO9837222A1] A method for the preparation of oligo- and/or polysaccharides, which are essentially homogenous with respect to the length of a carbohydrate chain and/or the respective molecular weight, from a sample having precursors of the oligo- and/or polysaccharide to be prepared, comprising oligo- and/or polysaccharides which are heterogenous with respect to the length of the carbohydrate chain and/or their respective molecular weight or do not have the appropriate length or weight, comprising the steps of: immobilizing precursors of the oligo- and/or polysaccharides with a carbohydrate chain present in a sample, by reversible binding the oligo- and/or polysaccharides on a surface having at least one domain for a specific interaction with said oligo- and/or polysaccharides, the at least one domain being capable of interacting at least with parts of the precursor of the oligo- and/or polysaccharide chain by contacting the sample with said surface and forming a complex between at least one domain and the precursor of the oligo- and/or polysaccharides; treating said complex with an agent for cleaving the carbohydrate chain selectively at the site of the domains; removing the cleaved product and release the oligo- and/or polysaccharides bound to the domain, said oligo- and/or polysaccharides having an essentially homogenous length of the carbohydrate chain or an essentially homogenous molecular weight.

IPC 1-7 (main, further and additional classification)

C12P 19/04; A61K 31/715; C07K 17/00; C08B 37/08; C08L 5/08; C12P 19/26; G01N 33/68

IPC 8 full level (invention and additional information)

A61K 31/715 (2006.01); C07K 14/47 (2006.01); C07K 17/00 (2006.01); C08B 37/08 (2006.01); C08L 5/08 (2006.01); C12P 19/04 (2006.01); C12P 19/26 (2006.01); G01N 33/66 (2006.01); G01N 33/68 (2006.01)

CPC (invention and additional information)

G01N 33/66 (2013.01); C07K 14/4725 (2013.01); C08B 37/0072 (2013.01); C12P 19/04 (2013.01); C12P 19/26 (2013.01); C07K 2319/00 (2013.01)

Citation (search report)

See references of WO 9837222A1

Designated contracting state (EPC)

AT BE CH DE ES FR GB IT LI LU NL SE

EPO simple patent family

WO 9837222 A1 19980827; EP 0861903 A1 19980902; EP 0972067 A1 20000119

INPADOC legal status

2002-06-26 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20011210

2000-06-14 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20000503

2000-01-26 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19990819

2000-01-19 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH DE ES FR GB IT LI LU NL SE