Global Patent Index - EP 0972069 A1

EP 0972069 A1 2000-01-19 - PRODUCTION OF FUNCTIONAL PROTEINS: BALANCE OF SHEAR STRESS AND GRAVITY

Title (en)

PRODUCTION OF FUNCTIONAL PROTEINS: BALANCE OF SHEAR STRESS AND GRAVITY

Title (de)

HERSTELLUNG FUNKTIONELLER PROTEINE: BALANCE VON SCHERBELASTUNG UND SCHWERKRAFT

Title (fr)

ELABORATION DE PROTEINES FONCTIONNELLES : EQUILIBRE ENTRE CONTRAINTE TANGENTIELLE ET GRAVITE

Publication

EP 0972069 A1 (EN)

Application

EP 98915320 A

Priority

  • US 9806826 W
  • US 4320597 P

Abstract (en)

[origin: WO9845468A1] The present invention provides a method for production of functional proteins including hormones by renal cells in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel. Natural mixture of renal cells expresses the enzyme 1-a-hydroxylase which can be used to generate the active form of vitamin D:1,25-diOH vitamin D3. The fibroblast cultures and co-culture of renal cortical cells express the gene for erythropoietin and secrete erythropoietin into the culture supernatant. Other shear stress response genes are also modulated by shear stress, such as toxin receptors megalin and cubulin (gp280). Also provided is a method of treating in-need individual with the functional proteins produced in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel.

IPC 1-7 (main, further and additional classification)

C12P 21/02; C07K 14/505; C07K 14/705; C12N 5/06; C12N 5/08; C12N 9/02; C12N 15/11

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12N 5/00 (2006.01); C12N 15/113 (2010.01); C12N 15/67 (2006.01); A61K 38/00 (2006.01)

CPC (invention and additional information)

C12N 15/113 (2013.01); C12N 5/0018 (2013.01); C12N 15/67 (2013.01); A61K 38/00 (2013.01); C12N 2310/13 (2013.01); C12N 2310/315 (2013.01); C12N 2501/60 (2013.01); C12N 2510/02 (2013.01)

Citation (search report)

See references of WO 9845468A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9845468 A1 19981015; AU 6953498 A 19981030; BR 9808510 A 20000523; CA 2286349 A1 19981015; EP 0972069 A1 20000119; IL 132264 D0 20010319; JP 2001517081 A 20011002

INPADOC legal status


2007-04-25 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20061103

2004-01-02 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20031111

2000-01-19 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19991021

2000-01-19 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE