EP 0972069 A1 20000119 - PRODUCTION OF FUNCTIONAL PROTEINS: BALANCE OF SHEAR STRESS AND GRAVITY
Title (en)
PRODUCTION OF FUNCTIONAL PROTEINS: BALANCE OF SHEAR STRESS AND GRAVITY
Title (de)
HERSTELLUNG FUNKTIONELLER PROTEINE: BALANCE VON SCHERBELASTUNG UND SCHWERKRAFT
Title (fr)
ELABORATION DE PROTEINES FONCTIONNELLES : EQUILIBRE ENTRE CONTRAINTE TANGENTIELLE ET GRAVITE
Publication
Application
Priority
- US 9806826 W 19980407
- US 4320597 P 19970408
Abstract (en)
[origin: WO9845468A1] The present invention provides a method for production of functional proteins including hormones by renal cells in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel. Natural mixture of renal cells expresses the enzyme 1-a-hydroxylase which can be used to generate the active form of vitamin D:1,25-diOH vitamin D3. The fibroblast cultures and co-culture of renal cortical cells express the gene for erythropoietin and secrete erythropoietin into the culture supernatant. Other shear stress response genes are also modulated by shear stress, such as toxin receptors megalin and cubulin (gp280). Also provided is a method of treating in-need individual with the functional proteins produced in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel.
IPC 1-7
C12P 21/02; C07K 14/705; C07K 14/505; C12N 9/02; C12N 15/11; C12N 5/06; C12N 5/08
IPC 8 full level
C12N 5/00 (2006.01); C12N 15/09 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12N 15/67 (2006.01); A61K 38/00 (2006.01)
CPC (source: EP)
C12N 5/0018 (2013.01); C12N 15/113 (2013.01); C12N 15/67 (2013.01); A61K 38/00 (2013.01); C12N 2310/13 (2013.01); C12N 2310/315 (2013.01); C12N 2501/60 (2013.01); C12N 2510/02 (2013.01)
Citation (search report)
See references of WO 9845468A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9845468 A1 19981015; AU 6953498 A 19981030; BR 9808510 A 20000523; CA 2286349 A1 19981015; EP 0972069 A1 20000119; IL 132264 A0 20010319; JP 2001517081 A 20011002
DOCDB simple family (application)
US 9806826 W 19980407; AU 6953498 A 19980407; BR 9808510 A 19980407; CA 2286349 A 19980407; EP 98915320 A 19980407; IL 13226498 A 19980407; JP 54098398 A 19980407