EP 0972074 A1 20000119 - A METHOD FOR IDENTIFYING THE SITE OF ACTION OF XENOBIOTIC CHEMICALS
Title (en)
A METHOD FOR IDENTIFYING THE SITE OF ACTION OF XENOBIOTIC CHEMICALS
Title (de)
EINE METHODE ZUR IDENTIFIZIERUNG DER WIRKUNGSSTELLEN XENOBIOTISCHER CHEMIKALIEN
Title (fr)
PROCEDE POUR IDENTIFIER LE SITE D'ACTION D'AGENTS CHIMIQUES XENOBIOTIQUES
Publication
Application
Priority
- US 9803684 W 19980226
- US 3958297 P 19970228
Abstract (en)
[origin: WO9838336A1] A method is disclosed for the discovery of the site of action of xenobiotic compounds. The method involves subjecting a detector cell comprising a genotoxic-sensitive promoter operably linked to a luminescent reporter gene complex. Exposure of the detector bacteria to compounds that are genotoxic results in an increase in luminescence. Such compounds are grouped and may be analyzed for pharmaceutical activity. Exposure of the detector bacteria to active xenobiotics that are not genotoxic results in inhibition of bioluminescence or stable luminescence. Subjection of the detector bacteria to a screen such as nutritional reversal or genetic titration reveals the site of action of the compound.
IPC 1-7
IPC 8 full level
C12N 1/21 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6897 (2018.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP)
C12Q 1/6897 (2013.01)
Citation (search report)
See references of WO 9838336A1
Designated contracting state (EPC)
BE CH DE DK ES FI FR GB IT LI NL PT SE
DOCDB simple family (publication)
WO 9838336 A1 19980903; AU 6339098 A 19980918; CA 2279576 A1 19980903; EP 0972074 A1 20000119; IL 131336 A0 20010128; JP 2001514501 A 20010911
DOCDB simple family (application)
US 9803684 W 19980226; AU 6339098 A 19980226; CA 2279576 A 19980226; EP 98907633 A 19980226; IL 13133698 A 19980226; JP 53781298 A 19980226