EP 0973805 A1 20000126 - METHOD FOR PRODUCTION OF ANTIBODIES TO SPECIFIC SITES OF RAPAMYCIN
Title (en)
METHOD FOR PRODUCTION OF ANTIBODIES TO SPECIFIC SITES OF RAPAMYCIN
Title (de)
VERFAHREN ZUR HERSTELLUNG VON ANTIKÖRPERN GEGEN SPEZIFISCHE STELLEN VON RAPAMYCIN
Title (fr)
PROCEDE PERMETTANT LA PRODUCTION D'ANTICORPS A DES SITES SPECIFIQUES DE LA RAPAMYCINE
Publication
Application
Priority
- CA 9800361 W 19980409
- US 4321597 P 19970409
Abstract (en)
[origin: WO9845333A1] This invention relates to the production of polyclonal and monoclonal antibodies to specific sites of rapamycin (Sirolimus). The reactivity of these poly and monoclonal antibodies make them particularly useful for immunoassays for therapeutic drug monitoring (TDM). These immunoassays or TDM kits may include polyclonal or monoclonal antibodies to specific sites of rapamycin. These kits may also include various combinations of polyclonal antibodies, polyclonal and monoclonal antibodies or a panel of monoclonal antibodies. Rapamycin conjugate immunogens are prepared for the immunization of a host animal to produce antibodies directed against specific regions of the rapamycin molecule. By determining the specific binding region of particular antibody, immunoassays which are capable of distinguishing between the parent molecule, active metabolites, inactive metabolites and other structurally similar immunosuppressant compounds are developed. The use of divinyl sulfone (DVS) as the linker arm molecule for forming rapamycin-protein conjugate immunogens is described. DVS-linked rapamycin-protein conjugates were found to elicit antibodies with greater specificity to the rapamycin molecule than succinate linked conjugates.
IPC 1-7
IPC 8 full level
C07K 14/435 (2006.01); C07K 14/765 (2006.01); C07K 16/12 (2006.01); C07K 16/14 (2006.01); C12N 5/10 (2006.01); C12N 15/02 (2006.01); C12P 21/08 (2006.01); G01N 33/53 (2006.01); G01N 33/577 (2006.01)
CPC (source: EP)
C07K 16/14 (2013.01)
Citation (search report)
See references of WO 9845333A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9845333 A1 19981015; AU 7020798 A 19981030; AU 740304 B2 20011101; CA 2286311 A1 19981015; EP 0973805 A1 20000126; JP 2001518784 A 20011016
DOCDB simple family (application)
CA 9800361 W 19980409; AU 7020798 A 19980409; CA 2286311 A 19980409; EP 98916710 A 19980409; JP 54219898 A 19980409