Global Patent Index - EP 0973805 A1

EP 0973805 A1 2000-01-26 - METHOD FOR PRODUCTION OF ANTIBODIES TO SPECIFIC SITES OF RAPAMYCIN

Title (en)

METHOD FOR PRODUCTION OF ANTIBODIES TO SPECIFIC SITES OF RAPAMYCIN

Title (de)

VERFAHREN ZUR HERSTELLUNG VON ANTIKÖRPERN GEGEN SPEZIFISCHE STELLEN VON RAPAMYCIN

Title (fr)

PROCEDE PERMETTANT LA PRODUCTION D'ANTICORPS A DES SITES SPECIFIQUES DE LA RAPAMYCINE

Publication

EP 0973805 A1 (EN)

Application

EP 98916710 A

Priority

  • CA 9800361 W
  • US 4321597 P

Abstract (en)

[origin: WO9845333A1] This invention relates to the production of polyclonal and monoclonal antibodies to specific sites of rapamycin (Sirolimus). The reactivity of these poly and monoclonal antibodies make them particularly useful for immunoassays for therapeutic drug monitoring (TDM). These immunoassays or TDM kits may include polyclonal or monoclonal antibodies to specific sites of rapamycin. These kits may also include various combinations of polyclonal antibodies, polyclonal and monoclonal antibodies or a panel of monoclonal antibodies. Rapamycin conjugate immunogens are prepared for the immunization of a host animal to produce antibodies directed against specific regions of the rapamycin molecule. By determining the specific binding region of particular antibody, immunoassays which are capable of distinguishing between the parent molecule, active metabolites, inactive metabolites and other structurally similar immunosuppressant compounds are developed. The use of divinyl sulfone (DVS) as the linker arm molecule for forming rapamycin-protein conjugate immunogens is described. DVS-linked rapamycin-protein conjugates were found to elicit antibodies with greater specificity to the rapamycin molecule than succinate linked conjugates.

IPC 1-7 (main, further and additional classification)

C07K 16/14; C12P 21/08; G01N 33/577

IPC 8 full level (invention and additional information)

G01N 33/53 (2006.01); C07K 14/435 (2006.01); C07K 14/765 (2006.01); C07K 16/12 (2006.01); C07K 16/14 (2006.01); C12N 5/10 (2006.01); C12N 15/02 (2006.01); C12P 21/08 (2006.01); G01N 33/577 (2006.01)

CPC (invention and additional information)

C07K 16/14 (2013.01)

Citation (search report)

See references of WO 9845333A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9845333 A1 19981015; AU 7020798 A 19981030; AU 740304 B2 20011101; CA 2286311 A1 19981015; EP 0973805 A1 20000126; JP 2001518784 A 20011016

INPADOC legal status


2006-04-19 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20051011

2004-01-28 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20031209

2000-01-26 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19991103

2000-01-26 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE