Global Patent Index - EP 0975652 A1

EP 0975652 A1 2000-02-02 - CAP-BINDING DOMAIN OF HUMAN EUKARYOTIC PROTEIN SYNTHESIS INITIATION FACTOR eIF-4E AND THE USE THEREOF

Title (en)

CAP-BINDING DOMAIN OF HUMAN EUKARYOTIC PROTEIN SYNTHESIS INITIATION FACTOR eIF-4E AND THE USE THEREOF

Title (de)

CAP-BINDENDE DOMAENE DES MENSCHLICHEN EUKARYOTISCHEN PROTEINSYNTHESE-INITIATIONSFAKTORS EIF-4E UND IHRE ANWENDUNG

Title (fr)

DOMAINE DE FIXATION DE COIFFE DU FACTEUR D'INITIATION eIF-4E DE SYNTHESE PROTEIQUE EUCARYOTE HUMAINE

Publication

EP 0975652 A1 (EN)

Application

EP 97942384 A

Priority

  • US 9715295 W
  • US 2488196 P
  • US 92087497 A

Abstract (en)

[origin: WO9808865A1] Binding of eIF-4E to the 5'm<7>G cap structure of eukaryotic mRNA signals the initiation of protein synthesis. In the present invention, the binding site cap-binding domain of eIF-4E was identified as the region containing the sequence Trp<113> to Arg<122>. Thus, in a first preferred embodiment the present invention provides a truncated peptide of the cap binding protein consisting essentially of the mRNA cap-binding domain. Specifically, the invention concerns eIF-4E cap binding peptide and its variants. In additional preferred embodiments, the invention provides an isolated nucleic acid molecule which encodes a truncated peptide of the eIF-4E protein consisting essentially of the mRNA cap-binding domain of eIF-4E; a recombinant vector and host cell comprising the above-described nucleic acid molecule; and a recombinant peptide produced thereby. The invention further provides the truncated or recombinant peptides immobilized on a solid support, a resin comprising the truncated or recombinant peptides immobilized on a solid support, methods and kits for isolating a capped mRNA molecule, and methods and kits for producing a full-length cDNA molecule.

IPC 1-7 (main, further and additional classification)

C07H 21/04; C07K 1/04; C07K 1/14; C07K 1/36; C07K 2/00; C07K 4/00; C07K 5/02; C07K 7/02

IPC 8 full level (invention and additional information)

C07K 14/47 (2006.01)

CPC (invention and additional information)

C07K 14/4705 (2013.01)

Citation (search report)

See references of WO 9808865A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9808865 A1 19980305; AU 4409497 A 19980319; EP 0975652 A1 20000202

INPADOC legal status


2002-11-06 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20020403

2000-02-02 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19990224

2000-02-02 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2000-02-02 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL PAYMENT 19990325;LT PAYMENT 19990325;LV PAYMENT 19990325;RO PAYMENT 19990325;SI PAYMENT 19990325