Global Patent Index - EP 0975768 A2

EP 0975768 A2 2000-02-02 - PHENOLIC ACID ESTERASE AND USE THEREOF

Title (en)

PHENOLIC ACID ESTERASE AND USE THEREOF

Title (de)

PHENOSÄURE ESTERASE UND VERWENDUNG

Title (fr)

ESTERASE D'ACIDE PHENOLIQUE ET UTILISATION DE CETTE DERNIERE

Publication

EP 0975768 A2 (EN)

Application

EP 98924104 A

Priority

  • EP 9802080 W
  • GB 9707540 A

Abstract (en)

[origin: GB2324302A] An enzyme, having phenolic acid esterase activity (preferably ferulic acid activity and/or coumaric acid esterase activity), which may be isolated from Piromyces spp. (preferably P.equi), is characterised by a pH optimum of greater than 6.5 and a temperature optimum greater than 45 degrees C. (as measured in a citric acid/disodium hydrogen orthophosphate buffer comprising 33 micromoles of FAXX (O-{5-O-[(E)-feruloyl]- alpha-L-arabinofuranosyl}-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose) as the substrate). DNA sequences encoding the enzyme, which may inserted into a vector and expressed in a transformed cell, are disclosed. The enzyme may in the form of a preparation having at least one further polysaccharide modifying and/or degrading enzyme, preferably a glycosyl hydrolase (especially xylanase, arabinase, alpha-L-arabinofuranosidase, endoglucanase, alpha-D-glucuronidase, pectinase, acetyl esterase, mannanase and acetyl xylan esterase). Such preparations may further comprise amylase, protease, alpha-galactosidase, phytase and/or lipase. The enzyme or enzyme preparation may be used in the preparation or release of phenolic acids from substrates comprising phenolic acid moieties, in the production of foodstuffs (including animal feed), in paper production, in the bioconversion of plant material or lignocellulose waste to sugars, and in the improvement of the digestibility of crop plants for livestock.

IPC 1-7 (main, further and additional classification)

C12N 15/55; A23K 1/165; C12N 9/18; C12P 7/42; C12P 19/00; D21H 17/00

IPC 8 full level (invention and additional information)

A23K 1/165 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/18 (2006.01); C12N 15/09 (2006.01); C12N 15/55 (2006.01); D21C 9/00 (2006.01); C12R 1/645 (2006.01)

CPC (invention and additional information)

C12N 9/18 (2013.01); A23K 20/189 (2016.05)

Citation (search report)

See references of WO 9846768A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

GB 2324302 A 19981021; GB 9707540 D0 19970604; AU 7642398 A 19981111; BR 9808554 A 20000523; CA 2286694 A1 19981022; CN 1255165 A 20000531; EP 0975768 A2 20000202; JP 2001523090 A 20011120; WO 9846768 A2 19981022; WO 9846768 A3 19990121

INPADOC legal status


2001-09-26 [18W] WITHDRAWN

- Date of withdrawal: 20010724

2000-02-02 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19991105

2000-02-02 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2000-02-02 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL PAYMENT 19991105;LT PAYMENT 19991105;LV PAYMENT 19991105;MK PAYMENT 19991105;RO PAYMENT 19991105;SI PAYMENT 19991105