Global Patent Index - EP 0975969 A1

EP 0975969 A1 2000-02-02 - METHODS FOR SCREENING COMPOUNDS USING ENCAPSULATED CELLS

Title (en)

METHODS FOR SCREENING COMPOUNDS USING ENCAPSULATED CELLS

Title (de)

SCREENING-METHODE FÜR VERBINDUNGEN MIT HILFE EINGEKAPSELTER ZELLEN

Title (fr)

METHODES DE TRI DE COMPOSES AU MOYEN DE CELLULES ENCAPSULEES

Publication

EP 0975969 A1 (EN)

Application

EP 98911863 A

Priority

  • US 9805462 W
  • US 4088897 P
  • US 7104698 P

Abstract (en)

[origin: WO9841869A1] The present invention relates to an integrated approach to drug screening that is designed to couple a screening assay both temporally and spatially to natural product synthesis in a microorganism. The present invention provides a screening unit which is a gel droplet comprising a producing species that produce natural products for the drug screen, and an assay system that detects or measures a desired biological activity. A producing species is coencapsulated with an assay system in a screening unit when the producing species is at a phase in its life cycle that is optimal for producing natural products, such as secondary metabolites. The producing species is spatially positioned relative to the assay system in the same unit such that compounds produced by the producing species can come into contact with the assay system. If a compound possesses the desired activity, the assay system will generate a signal that enables the identification and/or isolation of the screening unit. The present invention also provides methods for forming a screening unit, and methods for using a screening unit in drug screening. The methods are useful for screening naturally occurring organisms as well as genetically engineered cells, and solid carriers containing chemicals.

IPC 1-7 (main, further and additional classification)

G01N 33/549

IPC 8 full level (invention and additional information)

G01N 33/50 (2006.01)

CPC (invention and additional information)

G01N 33/502 (2013.01); G01N 33/5008 (2013.01); G01N 2333/36 (2013.01)

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9841869 A1 19980924; AU 6571598 A 19981012; CA 2284066 A1 19980924; EP 0975969 A1 20000202; EP 0975969 A4 20020731

INPADOC legal status


2004-04-07 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20031108

2002-07-31 [A4] SUPPLEMENTARY SEARCH REPORT

- Ref Legal Event Code: A4

- Effective date: 20020619

2002-07-31 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2002-07-31 [RIC1] CLASSIFICATION (CORRECTION)

- Ref Legal Event Code: RIC1

- Free Format Text: 7G 01N 33/549 A, 7G 01N 33/50 B

2000-02-02 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 19991007

2000-02-02 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE